HIV-1 Tropism and Survival in Vertically Infected Ugandan Infants

Jessica D CHURCH; Wei Huang; Neil PARKIN; Susan H ESHLEMAN; Anthony MWATHA; Jonathan TOMA; Eric STAWISKI; Deborah DONNELL; Laura A GUAY; Francis MMIRO; Philippa MUSOKE; J. Brooks JACKSON

doi:10.1086/587492

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HIV-1 Tropism and Survival in Vertically Infected Ugandan Infants

Journal article

Open access

Peer reviewed

HIV-1 Tropism and Survival in Vertically Infected Ugandan Infants

Jessica D CHURCH, Wei Huang, Neil PARKIN, Susan H ESHLEMAN, Anthony MWATHA, Jonathan TOMA, Eric STAWISKI, Deborah DONNELL, Laura A GUAY, Francis MMIRO, …

The Journal of infectious diseases, Vol.197(10), pp.1382-1388

2008

DOI: 10.1086/587492

PMID: 18444795

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Abstract

Background. Human immunodeficiency virus type 1 (HIV-1) may utilize the CXCR4 coreceptor (X4 virus), the CCR5 coreceptor (R5 virus), or both (dual/mixed [DM] virus). We analyzed HIV-1 coreceptor tropism in Ugandan infants enrolled in the HIVNET (HIV Network for Prevention Trials) 012 trial. Methods. Plasma or serum was analyzed using a commercial coreceptor tropism assay. HIV env subtype was determined by phylogenetic methods. Results. Tropism results were obtained for 57 samples from infants collected 6–14 weeks after birth. Fifty-two infants had only R5 virus, and 5 had either X4 or DM virus. The mothers of those 5 infants also had X4 or DM virus. In infants, subtype D infection was associated with high-level infectivity in CCR5-bearing cells and also with the detection of X4 or DM strains. High-level infectivity in CCR5-bearing cells was associated with decreased infant survival, but infection with X4 or DM virus was not. HIV clones from infants with DM viral populations showed different patterns of coreceptor use. V3 loop sequence- based algorithms predicted the tropism of some, but not all, env clones. Conclusions. Complex patterns of HIV tropism were found in HIV-infected newborn infants. Subtype D infection was associated with X4 virus and with high-level replication in CCR5-bearing cells. High-level replication of R5 virus was associated with decreased infant survival.

Infectious Diseases

Microbiology

Virology

Fundamental and applied biological sciences. Psychology

Miscellaneous

Biological and medical sciences

Medical sciences

Details

Title: Subtitle: HIV-1 Tropism and Survival in Vertically Infected Ugandan Infants
Creators: Jessica D CHURCH - Johns Hopkins University, Baltimore, Maryland, United States
Wei Huang - Monogram Biosciences, South San Francisco, California, United States
Neil PARKIN - Monogram Biosciences, South San Francisco, California, United States
Susan H ESHLEMAN - Johns Hopkins University, Baltimore, Maryland, United States
Anthony MWATHA - Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States
Jonathan TOMA - Monogram Biosciences, South San Francisco, California, United States
Eric STAWISKI - Monogram Biosciences, South San Francisco, California, United States
Deborah DONNELL - Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States
Laura A GUAY - Johns Hopkins University, Baltimore, Maryland, United States
Francis MMIRO - Makerere University-Johns Hopkins University (MUJHU) Research Collaboration, Uganda
Philippa MUSOKE - Makerere University, Kampala, Uganda
J. Brooks JACKSON - Johns Hopkins University, Baltimore, Maryland, United States
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.197(10), pp.1382-1388
DOI: 10.1086/587492
PMID: 18444795
NLM abbreviation: J Infect Dis
ISSN: 0022-1899
eISSN: 1537-6613
Publisher: University of Chicago Press; Chicago, IL
Language: English
Date published: 2008
Academic Unit: Pathology; VPMA - Administration
Record Identifier: 9984047625502771

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