Journal article
HIV-1 interactions with cells: from viral binding to cell-cell transmission
Current protocols in cell biology, Vol.Chapter 26(43), pp.Unit 26.5-Unit 26.5
06/2009
DOI: 10.1002/0471143030.cb2605s43
PMCID: PMC2692072
PMID: 19499507
Abstract
Characterization of HIV-1 interactions with host cells is critical for cell biology studies of HIV-1. This unit describes a set of methods and protocols to perform quantitative assays of HIV-1 binding, internalization, infection, and cell-cell transmission. The protocols include: (1) generating infectious single-cycle or replication-competent HIV-1 stocks, (2) an HIV-1 binding and internalization assay, (3) HIV-1 infection of target cells and quantification of viral infection, and (4) HIV-1 cell-cell transmission assays. These functional assays provide useful tools to quantitatively study HIV-1 infection and viral transmission.
Details
- Title: Subtitle
- HIV-1 interactions with cells: from viral binding to cell-cell transmission
- Creators
- Alicia M Janas - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin, USALi Wu
- Resource Type
- Journal article
- Publication Details
- Current protocols in cell biology, Vol.Chapter 26(43), pp.Unit 26.5-Unit 26.5
- DOI
- 10.1002/0471143030.cb2605s43
- PMID
- 19499507
- PMCID
- PMC2692072
- NLM abbreviation
- Curr Protoc Cell Biol
- ISSN
- 1934-2500
- eISSN
- 1934-2616
- Publisher
- United States
- Grant note
- R01 AI068493-03 / NIAID NIH HHS R01 AI068493 / NIAID NIH HHS R01 AI068493-04 / NIAID NIH HHS AI068493 / NIAID NIH HHS
- Language
- English
- Date published
- 06/2009
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984002376602771
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