Journal article
HIV-Induced Syncytia in Peripheral Blood Cell Cultures Crawl by Extending Giant Pseudopods
Cellular immunology, Vol.166(2), pp.261-274
12/1995
DOI: 10.1006/cimm.1995.9971
PMID: 7497528
Abstract
It was previously demonstrated that HIV-induced syncytia of the immortalized T cell line SupT1 reorganize their cytoskeleton and form a spherical supernuclear complex, thus mimicking the organization, polarity, and morphology of a single SupT1 cell. Then, through extension of a single, giant pseudopod, these syncytia, which grow to more than 100 times the volume of a single SupT1 cell, translocate along a substratum. To verify that syncytium motility is not peculiar to the SupT1 cell line, we have analyzed the cytoskeletal organization and motile capabilities of HIV-induced syncytia formed in peripheral blood cell cultures containing more than 90% CD4-positive cells. The results demonstrate that although peripheral blood T cells differ from SupT1 cells in size and morphology, they are continuously motile and translocate along a substratum in a manner quite similar to that of SupT1 cells, and peripheral blood T cell syncytia induced by HIV-1LAI as well as two additional clinical isolates translocate by the extension of a giant anterior pseudopod in a fashion indistinguishable from that of HIV-induced SupT1 syncytia. Together, these results support the generalization that HIV-induced T cell syncytia are motile.
Details
- Title: Subtitle
- HIV-Induced Syncytia in Peripheral Blood Cell Cultures Crawl by Extending Giant Pseudopods
- Creators
- Damon C Shutt - Department of Biological Sciences, University of Iowa and VA Medical Center, Iowa City, Iowa, 52242Jack T Stapleton - Department of Internal Medicine, University of Iowa and VA Medical Center, Iowa City, Iowa, 52242Ronald C Kennedy - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, 73190David R Soll - Department of Biological Sciences, University of Iowa and VA Medical Center, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.166(2), pp.261-274
- Publisher
- Elsevier Inc
- DOI
- 10.1006/cimm.1995.9971
- PMID
- 7497528
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 12/1995
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Biology; Nursing; Internal Medicine
- Record Identifier
- 9984094505602771
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