Journal article
HIV-induced syncytia of a T cell line form single giant pseudopods and are motile
Journal of cell science, Vol.106(3), pp.941-953
1993
DOI: 10.1242/jcs.106.3.941
PMID: 8308076
Abstract
The human immunodeficiency virus, HIV, induces syncytium formation in cultures of many T cell lines. These syncytia have previously been viewed as disorganized fusion products in the throes of death. Evidence is presented that in HIV1-infected SupT1 cultures, syncytia five times to over one hundred times larger than single cells organize their many nuclei into blastula-like balls, reorganize their cytoskeleton to mimic that of a single cell, and extend single, giant pseudopods in a polar fashion. Medium-sized syncytia are capable of translocation through extension of these giant pseudopods. The rate of translocation of syncytia is comparable to that of single cells. Single cell motility, syncytium motility and pseudopod extension also appear to play roles in the recruitment of cells into syncytia. Finally, condensation of F-actin at cell-syncytium and syncytium-syncytium adhesion sites suggests the involvement of the cytoskeleton in the adhesion and/or subsequent fusion event. These results suggest that the fusion events involved in HIV-induced syncytia formation involve both cell motility and reorganization of the cytoskeleton, and demonstrate that syncytia are highly organized, motile entities.
Details
- Title: Subtitle
- HIV-induced syncytia of a T cell line form single giant pseudopods and are motile
- Creators
- Andrew Sylwester - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United StatesDeborah Wessels - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United StatesStephanie A Anderson - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United StatesRonald Q Warren - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United StatesDamon C Shutt - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United StatesRonald C Kennedy - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United StatesDavid R Soll - Univ. Iowa, dep. biological sci., Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Journal of cell science, Vol.106(3), pp.941-953
- Publisher
- Company of Biologists; Cambridge
- DOI
- 10.1242/jcs.106.3.941
- PMID
- 8308076
- ISSN
- 0021-9533
- eISSN
- 1477-9137
- Language
- English
- Date published
- 1993
- Academic Unit
- Biology; Nursing
- Record Identifier
- 9984020740902771
Metrics
21 Record Views