HPLC analysis of putative amino acid neurotransmitters released from primary cerebellar cultures

Keith L Rogers; Robert A Philibert; Albert J Allen; Jerry Molitor; Elizabeth J Wilson; Gary R Dutton

doi:10.1016/0165-0270(87)90012-4

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HPLC analysis of putative amino acid neurotransmitters released from primary cerebellar cultures

Journal article

Peer reviewed

HPLC analysis of putative amino acid neurotransmitters released from primary cerebellar cultures

Keith L Rogers, Robert A Philibert, Albert J Allen, Jerry Molitor, Elizabeth J Wilson and Gary R Dutton

Journal of neuroscience methods, Vol.22(2), pp.173-179

1987

DOI: 10.1016/0165-0270(87)90012-4

PMID: 2893861

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Abstract

An HPLC method is described that measures amino acids (putative neurotransmitters and/or neuromodulators) released from primary, dissociated cerebellar cells maintained in monolayer culture. Precolumn derivatization with phenylisothiocyanate, followed by reverse phase chromatography with UV detection was used to quantitate the phenylcarbamyl amino acid derivatives in a chemically defined medium. Quantitation was linear, reproducible and sensitive to one picomole. This method is useful for the measurement of putative neurotransmitters GABA, glutamate, aspartate, taurine and adenosine, and can easily be modified to analyze other amino acids in physiological samples.

High performance liquid chromatography (HPLC)

Amino acid transmitter

Cerebellar cell culture

Details

Title: Subtitle: HPLC analysis of putative amino acid neurotransmitters released from primary cerebellar cultures
Creators: Keith L Rogers - Department of Psychiatry and Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
Robert A Philibert - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
Albert J Allen - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
Jerry Molitor - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
Elizabeth J Wilson - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
Gary R Dutton - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
Resource Type: Journal article
Publication Details: Journal of neuroscience methods, Vol.22(2), pp.173-179
DOI: 10.1016/0165-0270(87)90012-4
PMID: 2893861
NLM abbreviation: J Neurosci Methods
ISSN: 0165-0270
eISSN: 1872-678X
Publisher: Elsevier B.V
Language: English
Date published: 1987
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984003433302771

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