Journal article
HSP70 induction during exercise and heat stress in rats: role of internal temperature
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.268(1), pp.R92-R97
01/01/1995
DOI: 10.1152/ajpregu.1995.268.1.R92
PMID: 7840344
Abstract
The purpose of this study was to determine if the accumulation of the 72-kDa heat shock protein (HSP70) is elevated in response to a prolonged bout of submaximal exercise in which colonic temperature (Tco) remained at control levels. Sprague-Dawley rats were randomly assigned to one of four testing groups [n = 8 per group; ambient temperatures (Ta) for each condition are included]: 1) control (cool/rest; Ta = 24 degrees C); 2) cool and exercise (cool/exercise; Ta = 14 degrees C); 3) nonexertional heating (heat/rest; Ta = 42 degrees C); 4) heat and exercise (heat/exercise; Ta = 32 degrees C). All interventions were approximately 60 min in duration. An exercise bout consisted of treadmill running at 17 m/min and 0% grade, while the heat/rest and heat/exercise experiments consisted of heat exposure that was terminated when Tco reached 41 degrees C. Baseline Tco was similar for all four groups. In the cool/rest and cool/exercise groups, final Tco was not different from the baseline values, nor was it different between these two groups. In the heat/rest and heat/exercise groups, heating rates were similar. Tissue samples were obtained from the gastrocnemius, soleus, and extensor digitorum longus (EDL) muscles of the left hindlimb and the left ventricle 30 min after a trial was completed. An enzyme-linked immunosorbent assay specific for HSP70 was used to directly quantitate absolute levels of HSP70 in tissues. There were significant main effects of both heating and exercise for HSP70 levels in the gastrocnemius, soleus, and left ventricle (P < 0.05).
Details
- Title: Subtitle
- HSP70 induction during exercise and heat stress in rats: role of internal temperature
- Creators
- Rebecca Skidmore - Department of Exercise and Sport Sciences, Animal Sciences, Universityof Arizona, Tucson 85721Jesus A Gutierrez - Department of Exercise and Sport Sciences, Animal Sciences, Universityof Arizona, Tucson 85721Vince Guerriero Jr - Department of Exercise and Sport Sciences, Animal Sciences, Universityof Arizona, Tucson 85721Kevin C Kregel - Department of Exercise and Sport Sciences, Animal Sciences, Universityof Arizona, Tucson 85721
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.268(1), pp.R92-R97
- DOI
- 10.1152/ajpregu.1995.268.1.R92
- PMID
- 7840344
- ISSN
- 0363-6119
- eISSN
- 1522-1490
- Language
- English
- Date published
- 01/01/1995
- Academic Unit
- Radiation Oncology; Provost Office Administration; Health and Human Physiology
- Record Identifier
- 9984213451502771
Metrics
12 Record Views