Journal article
Habitually exercising older men do not demonstrate age-associated vascular endothelial oxidative stress
Aging cell, Vol.10(6), pp.1032-1037
10/17/2011
DOI: 10.1111/j.1474-9726.2011.00748.x
PMCID: PMC3222334
PMID: 21943306
Abstract
We tested the hypothesis that older men who perform habitual aerobic exercise do not demonstrate age-associated vascular endothelial oxidative stress compared with their sedentary peers. Older exercising men (n=13, 62±2 y) had higher (P<0.05) physical activity (79±7 vs. 30±6 MET h/wk) and maximal exercise oxygen consumption (42±1 vs. 29±1 ml/kg/min) vs. sedentary men (n=28, 63±1 y). Brachial artery flow-mediated dilation, a measure of vascular endothelial function, was greater (P<0.05) in the exercising vs. sedentary older men (6.3±0.5 vs. 4.9±0.4 %Δ) and not different than young controls (n=20, 25±1 y, 7.1 ± 0.5 %Δ). In vascular endothelial cells sampled from the brachial artery, nitrotyrosine, a marker of oxidative stress, was 51% lower in the exercising vs. sedentary older men (0.38±0.06 vs. 0.77±0.10 AU). This was associated with lower endothelial expression of the oxidant enzyme nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase (p47
phox
subunit, 0.33±0.05 vs. 0.61±0.09 AU) and the redox-sensitive transcription factor nuclear factor kappa B (NFκB) (p65 subunit, 0.36±0.05 vs. 0.72±0.09 AU). Expression of the antioxidant enzyme manganese superoxide dismutase (SOD) (0.57±0.13 vs. 0.30±0.04 AU) and activity of endothelium-bound extracellular SOD were greater (6.4±0.5 vs. 5.0±0.6 U/ml/min) in the exercising men (both P<0.05), but differences no longer were significant after correcting for adiposity and circulating metabolic factors. Overall, values for the young controls differed with those for the sedentary, but not the exercising older men. Older men who exercise regularly do not demonstrate vascular endothelial oxidative stress and this may be a key molecular mechanism underlying their reduced risk of cardiovascular diseases.
Details
- Title: Subtitle
- Habitually exercising older men do not demonstrate age-associated vascular endothelial oxidative stress
- Creators
- Gary L Pierce - University of Colorado BoulderAnthony J Donato - University of UtahThomas J LaRocca - University of Colorado BoulderIratxe Eskurza - University of Colorado BoulderAnnemarie E Silver - University of Colorado BoulderDouglas R Seals - University of Colorado Boulder
- Resource Type
- Journal article
- Publication Details
- Aging cell, Vol.10(6), pp.1032-1037
- DOI
- 10.1111/j.1474-9726.2011.00748.x
- PMID
- 21943306
- PMCID
- PMC3222334
- NLM abbreviation
- Aging Cell
- ISSN
- 1474-9718
- eISSN
- 1474-9726
- Grant note
- UL1 RR025780-02 || RR / National Center for Research Resources : NCRR R37 AG013038-10 || AG / National Institute on Aging : NIA R37 AG013038-12 || AG / National Institute on Aging : NIA T32 AG000279-08 || AG / National Institute on Aging : NIA R37 AG013038-11 || AG / National Institute on Aging : NIA UL1 RR025780-01 || RR / National Center for Research Resources : NCRR
- Language
- English
- Date published
- 10/17/2011
- Academic Unit
- Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984267137302771
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