Hair cell morphological patterns and polarity organization in the sea lamprey vestibular cristae

Bernd Fritzsch; Jennifer Kersigo; Kassidy Rejent; Wesley Gherman; Patrick W Frank; David R Giovannucci; Adel Maklad

doi:10.1002/ar.25164

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Hair cell morphological patterns and polarity organization in the sea lamprey vestibular cristae

Journal article

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Hair cell morphological patterns and polarity organization in the sea lamprey vestibular cristae

Bernd Fritzsch, Jennifer Kersigo, Kassidy Rejent, Wesley Gherman, Patrick W Frank, David R Giovannucci and Adel Maklad

Anatomical record (Hoboken, N.J. : 2007), Vol.306(8), pp.2170-2184

08/2023

DOI: 10.1002/ar.25164

PMID: 36651665

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Abstract

The inner ear of the sea lamprey was examined by scanning electron microscopy, antibody labeling with tubulin, Myo7a, Spectrin, and Phalloidin stain to elucidate the canal cristae organization and the morphology and polarity of the hair cells. We characterized the hair cell stereocilia bundles and their morphological polarity with respect to the kinocilia. We identified three types of hair cells. In Type 1 hair cells, the kinocilia were slightly longer than the tallest stereocilia. This type was located along the medial bank of the crista and their polarity, based on kinocilia location, was uniformly pointed ampullipetally. Type 2 hair cells that had kinocilia that were much longer than the stereocilia, were most abundant in the central region of the crista. This type of hair cell displayed variable polarity. Type 3 hair cells had extremely long kinocilia (~40-50 μm long) and with extremely short stereocilia. They were mostly located in the lateral zone crista and displayed ampullipetal polarity. Myo7a and tubulin antibodies revealed that hair cells and vestibular afferents are distributed across the canal cristae in the lamprey, covering the area of cruciate eminence; a feature that is absent in more derived vertebrates. Spectrin shows hair cells of varying polarities in the central zone. In this zone, some cells followed the main polarity vector (lateral) like those in medial and lateral zones, whereas other cells displayed polarities that carried up to 40° from the main polarity vector.

Evolution

labyrinth

balance

jawless vertebrate

vestibular system

Details

Title: Subtitle: Hair cell morphological patterns and polarity organization in the sea lamprey vestibular cristae
Creators: Bernd Fritzsch - University of Iowa
Jennifer Kersigo - University of Iowa
Kassidy Rejent - University of Toledo
Wesley Gherman - University of Toledo
Patrick W Frank - University of Toledo
David R Giovannucci - University of Toledo
Adel Maklad - University of Toledo
Resource Type: Journal article
Publication Details: Anatomical record (Hoboken, N.J. : 2007), Vol.306(8), pp.2170-2184
DOI: 10.1002/ar.25164
PMID: 36651665
NLM abbreviation: Anat Rec (Hoboken)
ISSN: 1932-8486
eISSN: 1932-8494
Grant note: R01 AG060504 / NIA NIH HHS R01 DC012060-01 / NIH HHS
Language: English
Electronic publication date: 01/18/2023
Date published: 08/2023
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984361748502771

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