Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone

G A Bishop; J A Frelinger

doi:10.1073/pnas.86.15.5933

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Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone

Journal article

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Peer reviewed

Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone

G A Bishop and J A Frelinger

Proceedings of the National Academy of Sciences - PNAS, Vol.86(15), pp.5933-5937

08/1989

DOI: 10.1073/pnas.86.15.5933

PMCID: PMC297745

PMID: 2788281

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Abstract

CH12.LX B cells are responsive to antigen-dependent differentiative signals transmitted through their surface Ek molecules. Although CH12.LX cells express surface Ak molecules with normal protein sequence, the Ak molecules do not deliver differentiative signal to these B cells. To determine whether introduction of a new A molecule into CH12.LX cells would correct this deficiency, CH12.LX cells were transfected with the genes encoding new Aalpha and/or Abeta molecules. It was found that transfected cells responded to antigen-specific signals delivered via the Ealphak Ebetak, Aalphab Abetab, or Aalphak Abetab molecule. However, the B cells did not respond to signals generated by the molecule Aalpha kAbetak, Aalphab Abetak, Aalphak Abetad, or Aalpha kAbetau. Comparison of these sequences suggests that two Abeta residues, His-47 and Trp-197, are important to the transmission of differentiative signals to B cells.

Details

Title: Subtitle: Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone
Creators: G A Bishop - Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599
J A Frelinger - Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.86(15), pp.5933-5937
DOI: 10.1073/pnas.86.15.5933
PMID: 2788281
PMCID: PMC297745
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 08/1989
Academic Unit: Microbiology and Immunology; President
Record Identifier: 9984001112402771

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