Journal article
Heat stress regulates the human 70-kDa heat-shock gene through the 3'-untranslated region
American journal of physiology. Lung cellular and molecular physiology, Vol.264(6), pp.L533-L537
06/01/1993
DOI: 10.1152/ajplung.1993.264.6.L533
PMID: 8333545
Abstract
Cells respond to a variety of stresses by synthesizing a family of proteins termed heat-shock proteins (HSP). Recently, the 3'-untranslated regions (UTRs) of some mRNAs have been shown to be important in the posttranscriptional regulation of protein production. Therefore, we hypothesized that heat could regulate HSP70 production through the HSP70 3'-UTR, in addition to its known effects on transcription. To test this hypothesis, cells were transfected with either a plasmid containing sequences encoding the human HSP70 or beta-globin 3'-untranslated region placed downstream of a chloramphenicol acetyltransferase (CAT) reporter gene. In both plasmids, the CAT gene was driven by an SV40 promoter. Following heat stress, cells transfected with the CAT construct containing the HSP70 3'-UTR showed increased CAT activity relative to the beta-globin 3'-UTR construct. This effect paralleled increases in HSP70 mRNA and levels of the inducible HSP70 protein by Western blot. These studies identify a heat-induced mechanism of posttranscriptional control of HSP70 synthesis utilizing the HSP70 3'-UTR, which may be important in the cells ability to regulate the heat-shock response.
Details
- Title: Subtitle
- Heat stress regulates the human 70-kDa heat-shock gene through the 3'-untranslated region
- Creators
- P. L. Moseley - University of IowaE. S. Wallen - University of IowaJ. D. McCafferty - University of IowaS. Flanagan - University of IowaJ. A. Kern - University of Iowa
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.264(6), pp.L533-L537
- DOI
- 10.1152/ajplung.1993.264.6.L533
- PMID
- 8333545
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Language
- English
- Date published
- 06/01/1993
- Academic Unit
- Health and Human Physiology
- Record Identifier
- 9984259397602771
Metrics
19 Record Views