Journal article
Helicobacter pylori: To Stain or Not to Stain?
American journal of clinical pathology, Vol.137(5), pp.733-738
2012
DOI: 10.1309/AJCP8DGTAVG7MBMT
PMID: 22523211
Abstract
We performed a retrospective study to investigate the usefulness of immunohistochemical stains for the diagnosis of Helicobacter pylori (HP). We reviewed 200 consecutive gastric biopsy specimens, as well as immunohistochemical stains for HP. Of the biopsy specimens, 32 were positive for HP by immunohistochemical staining; of those, HP was seen on H&E stains in 29 cases (91%). The number of high-power fields required to detect HP on H&E-stained slides ranged from 1 to 25 (mean, 5.75). Combined significant (2+ or 3+) acute and chronic inflammation had a specificity of 98% and a negative predictive value of 97%. Our results show that, in our institution, HP can be seen relatively easily with H&E staining in the majority of cases; however, a small number of cases with significant inflammation can be missed if stains are not used.
Details
- Title: Subtitle
- Helicobacter pylori: To Stain or Not to Stain?
- Creators
- S Brooks Smith - Department of Pathology, Wake Forest Baptist Health, Winston-Salem, NC, United StatesAnthony N SNOW - Department of Pathology, Wake Forest Baptist Health, Winston-Salem, NC, United StatesRandall L PERRY - Department of Pathology, Wake Forest Baptist Health, Winston-Salem, NC, United StatesShadi A QASEM - Department of Pathology, Wake Forest Baptist Health, Winston-Salem, NC, United States
- Resource Type
- Journal article
- Publication Details
- American journal of clinical pathology, Vol.137(5), pp.733-738
- Publisher
- American Society of Clinical Pathologists; Chicago, IL
- DOI
- 10.1309/AJCP8DGTAVG7MBMT
- PMID
- 22523211
- ISSN
- 0002-9173
- eISSN
- 1943-7722
- Language
- English
- Date published
- 2012
- Academic Unit
- Pathology
- Record Identifier
- 9984047706102771
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