Journal article
Helminth-induced regulation of T-cell transfer colitis requires intact and regulated T cell Stat6 signaling in mice
European journal of immunology, Vol.51(2), pp.433-444
02/2021
DOI: 10.1002/eji.201848072
PMID: 33067820
Abstract
Infection with parasitic worms (helminths) alters host immune responses and can inhibit pathogenic inflammation. Helminth infection promotes a strong Th2 and T regulatory response while suppressing Th1 and Th17 function. Th2 responses are largely dependent on transcriptional programs directed by Stat6-signaling. We examined the importance of intact T cell Stat6 signaling on helminth-induced suppression of murine colitis that results from T cell transfer into immune-deficient mice. Colonization with the intestinal nematode Heligmosomoides polygyrus bakeri resolves WT T cell transfer colitis. However, if the transferred T cells lack intact Stat6 then helminth exposure failed to attenuate colitis or suppress MLN T cell IFN-γ or IL17 production. Loss of Stat6 signaling resulted in decreased IL10 and increased IFN-γ co-expression by IL-17
T cells. We also transferred T cells from mice with constitutive T cell expression of activated Stat6 (Stat6VT). These mice developed a severe eosinophilic colitis that also was not attenuated by helminth infection. These results show that T cell expression of intact but regulated Stat6 signaling is required for helminth infection-associated regulation of pathogenic intestinal inflammation.
Details
- Title: Subtitle
- Helminth-induced regulation of T-cell transfer colitis requires intact and regulated T cell Stat6 signaling in mice
- Creators
- Ahmed Metwali - Iowa City VA Health Care SystemSarah Winckler - Iowa City VA Health Care SystemJoseph F Urban Jr - Beltsville Human Nutrition Research CenterMark H Kaplan - Indiana UniversityM Nedim Ince - Iowa City VA Health Care SystemDavid E Elliott - Iowa City VA Health Care System
- Resource Type
- Journal article
- Publication Details
- European journal of immunology, Vol.51(2), pp.433-444
- DOI
- 10.1002/eji.201848072
- PMID
- 33067820
- ISSN
- 0014-2980
- eISSN
- 1521-4141
- Grant note
- I01 BX002715 / BLRD VA
- Language
- English
- Date published
- 02/2021
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984359844302771
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