Journal article
Hemicholinium-3 derivatives A-4 and A-5 affect choline and acetylcholine metabolism
European journal of pharmacology. Molecular pharmacology section, Vol.206(2), pp.105-112
1991
DOI: 10.1016/0922-4106(91)90018-D
PMID: 2065716
Abstract
The neuroblastoma-glioma hybrid cell (NG108-15) has a sodium-dependent, high-affinity choline transport system with a K
m of 16.0 ± 3.4
μM and V
max of 214.5 ± 27.7 pmol/min/mg protein. A-4, A-5 and HC-3 produce dose-dependent inhibition of high-affinity choline transport in NG108-15 cells. Following 24 h exposure to approximately the EC
50 of each inhibitor, no significant decrease was found in total choline accumulation or in choline incorporation into phosphatidylcholine. However, when additional inhibitor was added during the 24 h incubation, significant decreases in choline accumulation were produced by A-4 and A-5. Following 24 h exposure to each compound, only A-4 was able to significantly affect free choline content. In contrast, each inhibitor was able to significantly decrease acetylcholine content following 24 h exposure. Possible reasons for consistent decreases in acetylcholine versus minimal changes in choline metabolism will be discussed.
Details
- Title: Subtitle
- Hemicholinium-3 derivatives A-4 and A-5 affect choline and acetylcholine metabolism
- Creators
- Kimberly Y. Sheff - Iowa Diabetes and Endocrinology Research CenterMark A. Yorek - Iowa Diabetes and Endocrinology Research CenterJohn P. Long - Iowa Diabetes and Endocrinology Research Center
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology. Molecular pharmacology section, Vol.206(2), pp.105-112
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0922-4106(91)90018-D
- PMID
- 2065716
- ISSN
- 0922-4106
- eISSN
- 1879-0712
- Language
- English
- Date published
- 1991
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359774402771
Metrics
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