Journal article
Hemodynamic and Hematologic Effects of Histotripsy of Free-Flowing Blood: Implications for Ultrasound-Mediated Thrombolysis
Journal of vascular and interventional radiology, Vol.26(10), pp.1559-1565
10/01/2015
DOI: 10.1016/j.jvir.2015.03.022
PMCID: PMC4584157
PMID: 25952642
Abstract
Purpose: To investigate the extent and consequences Of histotripsy-induced hemolysis in vivo.
Materials and Methods: Porcine femoral venous blood was treated with histotripsy in 11 animals with systemic heparinization and 11 without heparin. Serum and hemodynamic measurements were obtained at 0, 2, 5, 10, 15, and 30 minutes and 48-72 hours after the procedure. Fisher exact test Was used to determine differences in mortality between heparinized and nonheparinized groups. A linear mixed effects model was used to test for differences in blood analytes and hemodynamic variables over time.
Results: Of 11 animals in the nonheparinized group, 5 died during or immediately after histotripsy (45% nonheparin mortality vs 0% heparin mortality, P = .035). Serum hematocrit, free hemoglobin, lactate dehydrogenase (LDH), and right ventricular systolic pressure changed significantly (P < .001) over the treatment time. Serum hematocrit decreased slightly (from 32.5%+/- 3.6% to 29.4%+/- 4.2%), whereas increases were seen in free hemoglobin (from 6.2 mg/dL +/- 4.6 to 348 mg/dL +/- 100), LDH (from 365 U/L +/- 67.8 to 722 U/L +/- 84.7), and right ventricular systolic pressure (from 23.2 mm Hg +/- 7.2 to 39.7 mm Hg +/- 12.3). After 48-72 hours, hematocrit remained slightly decreased (P =.005), whereas LDH and free hemoglobin remained slightly increased compared with baseline (both P <.001).
Conclusions: Intravascular histotripsy applied to free-flowing venous blood is safe with systemic heparinization, causing only transient hemodynanuic and metabolic disturbances, supporting its use as a future noninvasive thrombolytic therapy modality.
Details
- Title: Subtitle
- Hemodynamic and Hematologic Effects of Histotripsy of Free-Flowing Blood: Implications for Ultrasound-Mediated Thrombolysis
- Creators
- Rajiv Devanagondi - Cleveland ClinicXi Zhang - University of MichiganZhen Xu - University of MichiganKimberly Ives - University of MichiganAlbert Levin - Henry Ford Health SystemHitinder Gurm - University of MichiganGabe E. Owens - University of Michigan
- Resource Type
- Journal article
- Publication Details
- Journal of vascular and interventional radiology, Vol.26(10), pp.1559-1565
- DOI
- 10.1016/j.jvir.2015.03.022
- PMID
- 25952642
- PMCID
- PMC4584157
- NLM abbreviation
- J Vasc Interv Radiol
- ISSN
- 1051-0443
- eISSN
- 1535-7732
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- Focused Ultrasound Foundation Strecher Fellow Award R21NS093121 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01EB008998 / National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) R01EB008998 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
- Language
- English
- Date published
- 10/01/2015
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984771656402771
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