Journal article
Hemodynamic effects of L- and D-S-nitroso-beta,beta-dimethylcysteine in rats
American journal of physiology. Heart and circulatory physiology, Vol.273(3), pp.H1493-H1501
09/01/1997
DOI: 10.1152/ajpheart.1997.273.3.H1493
PMID: 9321842
Abstract
This study examined whether S-nitroso-beta,beta-dimethylcysteine (S-nitrosopenicillamine; SNPEN) may activate stereoselective S-nitrosothiol receptors within the vasculature. We examined 1) the hemodynamic effects produced by the L- and D-isomers of SNPEN (12.5-400 nmol/kg iv), the L- and D-isomers of the parent thiols [L- and D-penicillamine (PEN); 12.5-400 nmol/kg iv], and the nitric oxide (NO) donor sodium nitroprusside (SNP; 1-10 micrograms/kg iv) in conscious rats; 2) the hemodynamic effects produced by these compounds in urethan-anesthetized rats; and 3) the relative decomposition of L- and D-SNPEN to NO on addition to rat blood or cultured porcine aortic smooth muscle (PASM) cells. We found that 1) L-SNPEN was a more potent hypotensive and vasodilator agent within the mesenteric bed and within sympathetically intact and sympathetically denervated hindlimb beds of conscious rats than was D-SNPEN; 2) the hypotension and vasodilation produced by L-SNPEN was similar in conscious and anesthetized rats, whereas the effects of D-SNPEN and SNP were augmented by urethan-anesthesia; 3) L- and D-PEN did not affect hemodynamic parameters in conscious or anesthetized rats; and 4) L- and D-SNPEN decomposed equally to NO on addition to rat blood or PASM cells. These results suggest that the vasodilator effects of SNPEN involve the interaction of this S-nitrosothiol with stereoselective recognition sites within the vasculature and that urethan alters the mechanisms by which L- and D-SNPEN relax vascular smooth muscle.
Details
- Title: Subtitle
- Hemodynamic effects of L- and D-S-nitroso-beta,beta-dimethylcysteine in rats
- Creators
- Mark D Travis - Cardiovascular Center, University of Iowa, Iowa City 52242, USARobin L Davisson - Cardiovascular Center, University of Iowa, Iowa City 52242, USAJames N Bates - Cardiovascular Center, University of Iowa, Iowa City 52242, USAStephen J Lewis - Cardiovascular Center, University of Iowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.273(3), pp.H1493-H1501
- DOI
- 10.1152/ajpheart.1997.273.3.H1493
- PMID
- 9321842
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 09/01/1997
- Academic Unit
- Anesthesia
- Record Identifier
- 9984007192302771
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