Heparin-dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2

Qingqing Yao; Yangxi Liu; Hongli Sun

doi:10.1002/term.2681

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Heparin-dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2

Journal article

Open access

Peer reviewed

Heparin-dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2

Qingqing Yao, Yangxi Liu and Hongli Sun

Journal of tissue engineering and regenerative medicine, Vol.12(6), pp.1519-1529

06/01/2018

DOI: 10.1002/term.2681

PMCID: PMC6013394

PMID: 29702734

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Abstract

The recently developed three-dimensional (3D) graphene foam (GrF) is intriguing for potential bone tissue engineering applications because it provides stem cells with a 3D porous substrate for osteogenic differentiation. However, the nature of graphene's structure lacks functional groups, thus making it difficult for further modification such as immobilization or conjugation of growth factors, which are normally required to promote tissue regeneration. To explore the potential of GrF functionalization and sustained release of therapeutic proteins, we fabricated a modified 3D GrF scaffold with bio-inspired heparin-dopamine (Hepa-Dopa) molecules using a highly scalable chemical vapour deposition method. Our data indicated that Hepa-Dopa modification resulted in significantly higher bone morphogenetic protein-2 (BMP2) binding ability and longer release capacity compared with the untreated scaffolds. Importantly, the heparin-functionalized 3D GrF significantly improved the exogenous BMP2-induced osteogenic differentiation. Therefore, our study, for the first time, indicated that the 3D GrF can be biomimetically functionalized with Hepa-Dopa and be used for sustained release of BMP2, thereby inducing osteogenic differentiation and suggesting promising potential as a new multifunctional carrier for therapeutic proteins and stem cells in bone tissue engineering.

Biotechnology & Applied Microbiology

Cell & Tissue Engineering

Cell Biology

Engineering

Engineering, Biomedical

Life Sciences & Biomedicine

Science & Technology

Technology

Details

Title: Subtitle: Heparin-dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2
Creators: Qingqing Yao - University of South Dakota
Yangxi Liu - University of South Dakota
Hongli Sun - University of South Dakota
Resource Type: Journal article
Publication Details: Journal of tissue engineering and regenerative medicine, Vol.12(6), pp.1519-1529
DOI: 10.1002/term.2681
PMID: 29702734
PMCID: PMC6013394
NLM abbreviation: J Tissue Eng Regen Med
ISSN: 1932-6254
eISSN: 1932-7005
Publisher: Wiley
Number of pages: 11
Grant note: DE027491 / NIH/NIDCRR03 LQ16H180003 / Zhejiang National Nature Science Foundations IIA-1335423 / National Science Foundation/EPSCoR; National Science Foundation (NSF) R03DE027491 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) 31600773 / Chinese National Nature Science Foundation; National Natural Science Foundation of China (NSFC)
Language: English
Date published: 06/01/2018
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
Record Identifier: 9984367740302771

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