Journal article
Hepatitis A Virus Attachment to Cultured Cell Lines
The Journal of infectious diseases, Vol.164(6), pp.1098-1103
12/1991
DOI: 10.1093/infdis/164.6.1098
PMID: 1659597
Abstract
Identification of a hepatitis A virus (HA V) receptor is important for understanding HAV tissue tropism and replication sites and in the design of vaccines and antiviral therapy. The attachment of HAV to cultured cell lines was evaluated: Calcium-dependent specific attachment of four HAV strains to permissive cells occurred, whereas binding to nonpermissive cells did not. Investigation of HAV antigenic variant strains (neutralization escape mutants) demonstrated identical attachment properties with neutralization-susceptible strains, suggesting that the immunodominant neutralization antigenic site of HAVis not directly involved in cell attachment. Unlike foot-and-mouth-disease virus, a related picornavirus, RGD peptides (arginine-glycineaspartic acid) were unable to interfere with HAV attachment. These studies demonstrate that HAV has a calcium-dependent receptor on cultured cell lines and suggest that the HAV binding region does not involve an RGD sequence or the HAV immunodominant neutralization site.
Details
- Title: Subtitle
- Hepatitis A Virus Attachment to Cultured Cell Lines
- Creators
- Jack T Stapleton - Division of Infectious Diseases, Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa CityJohn Frederick - Division of Infectious Diseases, Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa CityBarbara Meyer - Division of Infectious Diseases, Department of Internal Medicine, VA Medical Center and University of Iowa College of Medicine, Iowa City
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.164(6), pp.1098-1103
- Publisher
- The University of Chicago Press
- DOI
- 10.1093/infdis/164.6.1098
- PMID
- 1659597
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 12/1991
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094498102771
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