Journal article
Hepatitis C virus-core and non structural proteins lead to different effects on cellular antioxidant defenses
Journal of medical virology, Vol.76(4), pp.489-497
08/2005
DOI: 10.1002/jmv.20388
PMID: 15977232
Abstract
Chronic hepatitis C virus (HCV) infection leads to increased oxidative stress in the liver. Hepatic antioxidant enzymes provide an important line of defense against oxidative injury. To understand the antioxidant responses of hepatocytes to different HCV proteins, we compared changes in antioxidative enzymes in HCV-core and HCV-nonstructural protein expressing hepatocyte cell lines. We found that expression of HCV-core protein in hepatocyte cell lines leads to increased oxidative stress as determined by increased in the oxidant-sensitive probe 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-DCFH(2)) fluorescence, decreased reduced glutathione (GSH), and increased oxidation of thioredoxin (Trx). Although the expression of HCV-nonstructural (HCV-NS) proteins led to increased oxidative stress as well, the antioxidant enzymatic responses were different. Over-expression of HCV-NS proteins increased antioxidant enzymes (MnSOD and catalase), heme oxygenase-1 (HO-1), and GSH, indicating different mechanism(s) of prooxidative activity than HCV-core protein. Our findings show that different HCV proteins induce different antioxidant defense responses in hepatocytes. These findings may facilitate understanding the interaction of different HCV proteins with infected liver cells and help identify possible factors contributing to hepatocyte damage during HCV infection.
Details
- Title: Subtitle
- Hepatitis C virus-core and non structural proteins lead to different effects on cellular antioxidant defenses
- Creators
- Maher Y Abdalla - Department of Internal Medicine of the Roy G. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IowaIman M Ahmad - Department of Radiation Oncology, Free Radical and Radiation Biology Program, Iowa City, IowaDouglas R Spitz - Department of Radiation Oncology, Free Radical and Radiation Biology Program, Iowa City, IowaWarren N Schmidt - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, Iowa City, IowaBradley E Britigan - Department of Internal Medicine and Research Service, Veterans Administration Medical Center, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of medical virology, Vol.76(4), pp.489-497
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jmv.20388
- PMID
- 15977232
- ISSN
- 0146-6615
- eISSN
- 1096-9071
- Number of pages
- 9
- Language
- English
- Date published
- 08/2005
- Academic Unit
- Gastroenterology and Hepatology; Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984094874802771
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