Journal article
Herpes Simplex Virus 1 pUL34 Plays a Critical Role in Cell-to-Cell Spread of Virus in Addition to Its Role in Virus Replication
Journal of virology, Vol.85(14), pp.7203-7215
07/2011
DOI: 10.1128/JVI.00262-11
PMCID: PMC3126596
PMID: 21561917
Abstract
Herpes simplex virus (HSV) pUL34 plays a critical role in virus replication by mediating egress of nucleocapsids from the infected cell nucleus. We have identified a mutation in pUL34 (Y68A) that produces a major defect in virus replication and impaired nuclear egress but also profoundly inhibits cell-to-cell spread and trafficking of gE. Virion release to the extracellular medium is not affected by the Y68A mutation, indicating that the mutation specifically inhibits cell-to-cell spread. We isolated extragenic suppressors of the Y68A plaque formation defect and mapped them by a combination of high-throughput Illumina sequencing and PCR-based screening. We found that suppression is highly correlated with a nonsense mutation in the US9 gene, which plays a critical role in cell-to-cell spread of HSV-1 in neurons. The US9 mutation alone is not sufficient to suppress the Y68A spread phenotype, indicating a likely role for multiple viral factors.
Details
- Title: Subtitle
- Herpes Simplex Virus 1 pUL34 Plays a Critical Role in Cell-to-Cell Spread of Virus in Addition to Its Role in Virus Replication
- Creators
- Alison C Haugo - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242Moriah L Szpara - Department of Molecular Biology and the Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey 08544Lance Parsons - Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544Lynn W Enquist - Department of Molecular Biology and the Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey 08544Richard J Roller - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.85(14), pp.7203-7215
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- DOI
- 10.1128/JVI.00262-11
- PMID
- 21561917
- PMCID
- PMC3126596
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 07/2011
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001204802771
Metrics
20 Record Views