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Herpes simplex virus type 1 U(L)34 gene product is required for viral envelopment
Journal article   Open access   Peer reviewed

Herpes simplex virus type 1 U(L)34 gene product is required for viral envelopment

Richard J Roller, Yuping Zhou, Renee Schnetzer, John Ferguson and Diana DeSalvo
Journal of virology, Vol.74(1), pp.117-129
01/2000
DOI: 10.1128/JVI.74.1.117-129.2000
PMCID: PMC111520
PMID: 10590098
url
https://doi.org/10.1128/JVI.74.1.117-129.2000View
Published (Version of record) Open Access

Abstract

The herpes simplex virus type 1 U(L)34 gene encodes a protein that is conserved in all human herpesviruses. The association of the U(L)34 protein with membranes in the infected cell and its expression as a gamma-1 gene suggest a role in maturation or egress of the virus particle from the cell. To determine the function of this gene product, we have constructed a recombinant virus that fails to express the U(L)34 protein. This recombinant virus, in which the U(L)34 protein coding sequence has been replaced by green fluorescent protein, forms minute plaques and replicates in single-step growth experiments to titers 3 to 5 log orders of magnitude lower than wild-type or repair viruses. On Vero cells, the deletion virus synthesizes proteins of all kinetic classes in normal amounts. Electron microscopic and biochemical analyses show that morphogenesis of the deletion virus proceeds normally to the point of formation of DNA-containing nuclear capsids, but electron micrographs show no enveloped virus particles in the cytoplasm or at the surface of infected cells, suggesting that the U(L)34 protein is essential for efficient envelopment of capsids.
 Cell Line Sequence Deletion Herpesvirus 1, Human - physiology Animals Humans Viral Proteins - physiology Cercopithecus aethiops Viral Proteins - genetics Herpesvirus 1, Human - genetics Microscopy, Electron Vero Cells

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