Heterochromatic silencing of Drosophila heat shock genes acts at the level of promoter potentiation

Diane E. Cryderman; Hongbing Tang; Christine Bell; David S. Gilmour; Lori L. Wallrath

doi:10.1093/nar/27.16.3364

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Heterochromatic silencing of Drosophila heat shock genes acts at the level of promoter potentiation

Journal article

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Heterochromatic silencing of Drosophila heat shock genes acts at the level of promoter potentiation

Diane E. Cryderman, Hongbing Tang, Christine Bell, David S. Gilmour and Lori L. Wallrath

Nucleic acids research, Vol.27(16), pp.3364-3370

08/1999

DOI: 10.1093/nar/27.16.3364

PMCID: PMC148571

PMID: 10454645

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Abstract

In a variety of organisms, genes placed near hetero-chromatin are transcriptionally silenced. In order to understand the molecular mechanisms responsible for this block in transcription, high resolution in vivo chromatin structure analysis was performed on two heat shock genes, hsp26 and hsp70. These genes normally reside in euchromatin where GAGA factor and RNA Pol II are present on the promoter prior to heat shock induction. P-element transformation experiments led to the identification of stocks in which these two genes were inserted within heterochromatin of the chromosome 4 telomeric region. These transgenes exhibit silencing that is partially suppressed by mutations in the gene encoding HP1. Micrococcal nuclease analysis revealed that the heterochromatic transgenes were packaged in a more regular nucleosome array than when located in euchromatin. High resolution DNase I analysis demonstrated that GAGA factor and TFIID were not associated with these promoters in heterochromatin; potassium permanganate experiments showed a loss of Pol II association. Taken together, these data suggest that occlusion of transacting factors from their cis-acting regulatory elements leading to a block in promoter potentiation is a mechanism for heterochromatin gene silencing.

Details

Title: Subtitle: Heterochromatic silencing of Drosophila heat shock genes acts at the level of promoter potentiation
Creators: Diane E. Cryderman - University of Iowa
Hongbing Tang - Pennsylvania State University
Christine Bell - Pennsylvania State University
David S. Gilmour - Pennsylvania State University
Lori L. Wallrath - University of Iowa
Resource Type: Journal article
Publication Details: Nucleic acids research, Vol.27(16), pp.3364-3370
DOI: 10.1093/nar/27.16.3364
PMID: 10454645
PMCID: PMC148571
NLM abbreviation: Nucleic Acids Res
ISSN: 0305-1048
eISSN: 1362-4962
Publisher: Oxford University Press
Language: English
Date published: 08/1999
Academic Unit: Biochemistry and Molecular Biology; University College Courses
Record Identifier: 9984288716702771

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