Journal article
Heterochromatin protein 1a is required for an open chromatin structure
Transcription, Vol.2(2), pp.95-99
2011
DOI: 10.4161/trns.2.2.14687
PMCID: PMC3062402
PMID: 21468237
Abstract
The
Drosophila melanogaster
fourth chromosome contains interspersed domains of active and repressive chromatin. We investigated a stock harboring a silenced transgene inserted into
Dyrk3
and near
Caps
—two expressed genes on chromosome four. In an HP1a-deficient background, transgene expression was activated while, paradoxically, expression of
Dyrk3
and
Caps
was reduced. We found that the promoters of
Dyrk3
and
Caps
contained DNase I hypersensitive sites but also possessed methylated histone H3 and HP1a, marks of repressive chromatin. In HP1a-deficient flies, the
Dyrk3
and
Caps
promoters displayed diminished accessibility to nuclease digestion, revealing a surprising role for HP1a in opening chromatin.
Details
- Title: Subtitle
- Heterochromatin protein 1a is required for an open chromatin structure
- Creators
- Diane E Cryderman - Department of Biochemistry; University of Iowa; Iowa City, IA USAMichael W Vitalini - Department of Biochemistry; University of Iowa; Iowa City, IA USALori L Wallrath - Department of Biochemistry; University of Iowa; Iowa City, IA USA
- Resource Type
- Journal article
- Publication Details
- Transcription, Vol.2(2), pp.95-99
- DOI
- 10.4161/trns.2.2.14687
- PMID
- 21468237
- PMCID
- PMC3062402
- NLM abbreviation
- Transcription
- ISSN
- 2154-1264
- eISSN
- 2154-1272
- Publisher
- Landes Bioscience
- Language
- English
- Date published
- 2011
- Academic Unit
- Biochemistry and Molecular Biology; University College Courses
- Record Identifier
- 9984024561602771
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