Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin

Christopher D Shaffer; Gena E Stephens; Brandi A Thompson; Levi Funches; John A Bernat; Carolyn A Craig; Sarah C. R Elgin

doi:10.1073/pnas.212458899

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Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin

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Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin

Christopher D Shaffer, Gena E Stephens, Brandi A Thompson, Levi Funches, John A Bernat, Carolyn A Craig and Sarah C. R Elgin

Proceedings of the National Academy of Sciences - PNAS, Vol.99(22), pp.14332-14337

10/29/2002

DOI: 10.1073/pnas.212458899

PMCID: PMC137884

PMID: 12376620

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Abstract

Heterochromatin protein 1 (HP1), first discovered in Drosophila melanogaster , is a highly conserved chromosomal protein implicated in both heterochromatin formation and gene silencing. We report here characterization of an HP1-interacting protein, heterochromatin protein 2 (HP2), which codistributes with HP1 in the pericentric heterochromatin. HP2 is a large protein with two major isoforms of approximately 356 and 176 kDa. The smaller isoform is produced from an alternative splicing pattern in which two exons are skipped. Both isoforms contain the domain that interacts with HP1; the larger isoform contains two AT-hook motifs. Mutations recovered in HP2 act as dominant suppressors of position effect variegation, confirming a role in heterochromatin spreading and gene silencing.

Biological Sciences

Details

Title: Subtitle: Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin
Creators: Christopher D Shaffer - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
Gena E Stephens - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
Brandi A Thompson - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
Levi Funches - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
John A Bernat - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
Carolyn A Craig - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
Sarah C. R Elgin - Department of Biology, Washington University, CB-1229, St. Louis, MO 63130
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.99(22), pp.14332-14337
DOI: 10.1073/pnas.212458899
PMID: 12376620
PMCID: PMC137884
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 10/29/2002
Academic Unit: Stead Family Department of Pediatrics; Medical Genetics and Genomics
Record Identifier: 9984093219002771

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