Journal article
Heterodimerization Between the Lutropin and Follitropin Receptors is Associated With an Attenuation of Hormone-Dependent Signaling
Endocrinology (Philadelphia), Vol.154(10), pp.3925-3930
10/01/2013
DOI: 10.1210/en.2013-1407
PMCID: PMC3776865
PMID: 23825122
Abstract
The LH receptor (LHR) and FSH receptor (FSHR) are each G protein-coupled receptors that play critical roles in reproductive endocrinology. Each of these receptors has previously been shown to self-associate into homodimers and oligomers shortly after their biosynthesis. As shown herein using bioluminescence resonance energy transfer to detect protein-protein interactions, our data show that the LHR and FSHR, when coexpressed in the same cells, specifically heterodimerize with each other. Further experiments confirm that at least a portion of the cellular LHR/FSHR heterodimers are present on the cell surface and are functional. We then sought to ascertain what effects, if any, heterodimerization between the LHR and FSHR might have on signaling. It was observed that when the LHR was expressed under conditions promoting the heterodimerization with FSHR, LH or human chorionic gonadotropin (hCG) stimulation of Gs was attenuated. Conversely, when the FSHR was expressed under conditions promoting heterodimerization with the LHR, FSH-stimulated Gs activation was attenuated. These results demonstrate that the coexpression of the LHR and FSHR enables heterodimerizaton between the 2 gonadotropin receptors and results in an attenuation of signaling through each receptor.
Details
- Title: Subtitle
- Heterodimerization Between the Lutropin and Follitropin Receptors is Associated With an Attenuation of Hormone-Dependent Signaling
- Creators
- Xiuyan Feng - Department of Molecular Biophysics and Physiology, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa 52242Meilin Zhang - Department of Molecular Biophysics and Physiology, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa 52242Rongbin Guan - Department of Molecular Biophysics and Physiology, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa 52242Deborah L Segaloff - Department of Molecular Biophysics and Physiology, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Endocrinology (Philadelphia), Vol.154(10), pp.3925-3930
- DOI
- 10.1210/en.2013-1407
- PMID
- 23825122
- PMCID
- PMC3776865
- ISSN
- 0013-7227
- eISSN
- 1945-7170
- Language
- English
- Date published
- 10/01/2013
- Academic Unit
- Molecular Physiology and Biophysics; Obstetrics and Gynecology; Medicine Administration
- Record Identifier
- 9984083806802771
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