Journal article
Heterogeneity of group A streptococcal pyrogenic exotoxin type B
Infection and immunity, Vol.20(2), pp.512-518
05/1978
DOI: 10.1128/IAI.20.2.512-518.1978
PMCID: PMC421884
PMID: 352946
Abstract
Streptococcal pyrogenic exotoxin type B purified from culture filtrates of either the NY-5 or T-19 strain of group A streptococcus was found to be heterogeneous in charge. Three protein fractions with isoelectric points of 8.0, 8.4, and 9.0 were isolated by differential solubility in ethanol and acetate-buffered saline followed by isoelectric focusing and shown to be antigenically identical to streptococcal pyrogenic exotoxin type B. The molecular weights of all three fractions were approximately 17,500, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with aggregates forming in the presence of hyaluronic acid. Only the pI 8.4 fraction showed the characteristic activities of streptococcal pyrogenic exotoxin in rabbits: pyrogenicity and ability to enhance susceptibility to lethal endotoxin shock. The pI 8.0 and pI 9.0 fractions were not pyrogenic, but could be used to immunize against pyrogenicity. These two fractions failed either to enhance lethal endotoxin shock or to immunize against enhancement activity. When the isolated fractions were electrofocused again they appeared heterogeneous, suggesting an instability of the B toxin molecular forms.
Details
- Title: Subtitle
- Heterogeneity of group A streptococcal pyrogenic exotoxin type B
- Creators
- E L BarsumianC M CunninghamP M SchlievertD W Watson
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.20(2), pp.512-518
- Publisher
- United States
- DOI
- 10.1128/IAI.20.2.512-518.1978
- PMID
- 352946
- PMCID
- PMC421884
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 05/1978
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001113102771
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