Journal article
Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression
The Journal of biological chemistry, Vol.269(8), pp.6040-6044
1994
DOI: 10.1016/S0021-9258(17)37566-X
PMID: 8119949
Abstract
The 59-kDa dystrophin-associated protein triplet (59-DAP) is a component of the dystrophin-glycoprotein complex which may directly associate with dystrophin. The cDNA encoding one component (59-1 DAP) of the 59-DAP triplet has now been cloned from rabbit skeletal muscle. The deduced amino acid sequence of 59-1 DAP predicts a 505-amino acid polypeptide containing nine potential phosphorylation sites and no predicted transmembrane domains. This is consistent with the 59-1 DAP being a peripheral membrane protein associated with the cytoplasmic face of the dystrophin-glycoprotein complex. Affinity-purified antibodies against rabbit 59-1 DAP fusion proteins only recognize the lowest band of the 59-DAP triplet in skeletal muscle sarcolemma and isolated dystrophin-glycoprotein complex. The tissue-specific expression of 59-1 DAP mRNA, which is most prominent in skeletal and cardiac muscle and is also detected in brain, parallels that of dystrophin but not of utrophin. Levels of 59-1 DAP mRNA are unaffected in mdx mouse skeletal and cardiac muscles, although all dystrophin-associated proteins, including 59-DAP, are greatly reduced in mdx mouse skeletal muscle. However, in mdx mouse cardiac muscle, the up-regulation of utrophin preserves all dystrophin-associated proteins except 59-DAP. Our results suggest that the 59-DAP triplet may contain different protein species and that the 59-1 DAP may associate more specifically with dystrophin than with utrophin.
Details
- Title: Subtitle
- Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression
- Creators
- BIN Yang - Howard Hughes medical inst., Iowa City IA 52242, United StatesO IBRAGHIMOV-BESKROVNAYA - Howard Hughes medical inst., Iowa City IA 52242, United StatesC. R Moomaw - Howard Hughes medical inst., Iowa City IA 52242, United StatesC. A Slaughter - Howard Hughes medical inst., Iowa City IA 52242, United StatesK. P Campbell - University of Iowa, Molecular Physiology and Biophysics
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.269(8), pp.6040-6044
- DOI
- 10.1016/S0021-9258(17)37566-X
- PMID
- 8119949
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- American Society for Biochemistry and Molecular Biology
- Language
- English
- Date published
- 1994
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068369502771
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