Journal article
Heteronuclear NMR studies of 13C-labeled yeast cell wall ?-glucan oligosaccharides
Journal of biomolecular NMR, Vol.3(4), 429
07/1993
DOI: 10.1007/BF00176009
Abstract
The structures of uniformly 13C-labeled β-glucan octa- and undeca-oligosaccharides enzymatically prepared by the yeast cell wall glucanosyl transferase of Candida albicans were characterized by using a combination of HCCH-COSY, HCCH-TOCSY, and HMBC experiments. The oligosaccharide structures indicate that the cell wall glucanosyl transferase cleaves two glucosyl units from the reducing end of the initial linear β(1→3) penta-oligosaccharide and subsequently transfers the remainder to another oligosaccharide at the nonreducing end via a β(1→6) linkage. These results indicate that the combined action of cell wall glucanase and glucanosyl transferase activities could not only introduce intrachain β(1→6) linkages within a single glucan strand, but also result in cross-linking of two initially separate glucan strands with concurrent introduction of intrachain β(1→6) linkages. Since isolated fungal membranes only synthesize linear β(1→3) glucan strands, wall-associated enzymes probably participate in the assembly of the final wall glucan structure during cell growth and division.
Details
- Title: Subtitle
- Heteronuclear NMR studies of 13C-labeled yeast cell wall ?-glucan oligosaccharides
- Creators
- Liping Yu - Discovery LaboratoriesRobert Goldman - Discovery LaboratoriesPatrick SullivanGregF WalkerStephenW Fesik
- Resource Type
- Journal article
- Publication Details
- Journal of biomolecular NMR, Vol.3(4), 429
- DOI
- 10.1007/BF00176009
- ISSN
- 0925-2738
- eISSN
- 1573-5001
- Language
- English
- Date published
- 07/1993
- Academic Unit
- Biochemistry and Molecular Biology; Medicine Administration
- Record Identifier
- 9984627252302771
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