Journal article
Hierarchical Interactions of Homeodomain and Forkhead Transcription Factors in Regulating Odontogenic Gene Expression
The Journal of biological chemistry, Vol.286(24), pp.21372-21383
06/17/2011
DOI: 10.1074/jbc.M111.252031
PMCID: PMC3122197
PMID: 21504905
Abstract
FoxJ1 is a forkhead transcription factor expressed in multiple tissues during development and a major regulator of cilia development.
FoxJ1
−/−
mice present with defects in odontogenesis, and we correlate these defects to hierarchical interactions between homeodomain factors Pitx2 and Dlx2 with FoxJ1 in regulating their expression through direct physical interactions. Chromatin immunoprecipitation assays reveal endogenous Pitx2 and Dlx2 binding to the
Dlx2
promoter and Dlx2 binding to the
FoxJ1
promoter as well as Dlx2 and FoxJ1 binding to the
amelogenin
promoter. PITX2 activation of the
Dlx2
promoter is attenuated by a direct Dlx2 physical interaction with PITX2. Dlx2 autoregulates its promoter, and Dlx2 transcriptionally activates the downstream gene
FoxJ1.
Dlx2 and FoxJ1 physically interact and synergistically regulate both
Dlx2
and
FoxJ1
promoters. Dlx2 and FoxJ1 also activate the
amelogenin
promoter, and amelogenin is required for enamel formation and late stage tooth development.
FoxJ1
−/−
mice maxillary and mandibular incisors are reduced in length and width and have reduced amelogenin expression.
FoxJ1
−/−
mice show a reduced and defective ameloblast layer, revealing a biological effect of these transcription factor hierarchies during tooth morphogenesis. These transcriptional mechanisms may contribute to other developmental processes such as neuronal, pituitary, and heart development.
Details
- Title: Subtitle
- Hierarchical Interactions of Homeodomain and Forkhead Transcription Factors in Regulating Odontogenic Gene Expression
- Creators
- Shankar R Venugopalan - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Xiao Li - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Melanie A Amen - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Sergio Florez - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Diana Gutierrez - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Huojun Cao - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Jianbo Wang - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030Brad A Amendt - From the Texas A&M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.286(24), pp.21372-21383
- DOI
- 10.1074/jbc.M111.252031
- PMID
- 21504905
- PMCID
- PMC3122197
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- American Society for Biochemistry and Molecular Biology; 9650 Rockville Pike, Bethesda, MD 20814, U.S.A
- Grant note
- DE 18885 / National Institutes of Health
- Alternative title
- FoxJ1 Interactions with Homeodomain Factors
- Language
- English
- Date published
- 06/17/2011
- Academic Unit
- Orthodontics; Anatomy and Cell Biology; Endodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984066099402771
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