Journal article
High Frequency of Virus-Specific Interleukin-2-Producing CD4+ T Cells and Th1 Dominance during Lymphocytic Choriomeningitis Virus Infection
Journal of virology, Vol.74(9), pp.4429-4432
Note
05/2000
DOI: 10.1128/JVI.74.9.4429-4432.2000
PMCID: PMC111961
PMID: 10756059
Abstract
Analysis of C57BL/6 mice acutely infected with lymphocytic choriomeningitis virus (LCMV) by using intracellular cytokine staining revealed a high frequency (2 to 10%) of CD4
+
T cells secreting the Th1-associated cytokines interleukin-2 (IL-2), gamma interferon (IFN-γ), and tumor necrosis factor alpha, with no concomitant increase in the frequency of CD4
+
T cells secreting the Th2-associated cytokines IL-4, IL-5, and IL-10 following stimulation with viral peptides. In LCMV-infected C57BL/6 CD8
−/−
mice, more than 20% of the CD4
+
T cells secreted IFN-γ after viral peptide stimulation, whereas less than 1% of the CD4
+
T cells secreted IL-4 under these same conditions. Mice persistently infected with a high dose of LCMV clone 13 also generated a virtually exclusive Th1 response. Thus, LCMV induces a much more profound virus-specific CD4
+
T-cell response than previously recognized, and it is dramatically skewed to a Th1 phenotype.
Details
- Title: Subtitle
- High Frequency of Virus-Specific Interleukin-2-Producing CD4+ T Cells and Th1 Dominance during Lymphocytic Choriomeningitis Virus Infection
- Creators
- Steven M Varga - Program in Immunology and VirologyRaymond M Welsh - Program in Immunology and Virology
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.74(9), pp.4429-4432
- Series
- Note
- DOI
- 10.1128/JVI.74.9.4429-4432.2000
- PMID
- 10756059
- PMCID
- PMC111961
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 05/2000
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083804702771
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