Journal article
High Prevalence of Staphylococcus aureus Enterotoxin Gene Cluster Superantigens in Cystic Fibrosis Clinical Isolates
Genes, Vol.10(12), p.1036
12/01/2019
DOI: 10.3390/genes10121036
PMCID: PMC6947208
PMID: 31842331
Abstract
Background: Staphylococcus aureus is a highly prevalent respiratory pathogen in cystic fibrosis (CF). It is unclear how this organism establishes chronic infections in CF airways. We hypothesized that S. aureus isolates from patients with CF would share common virulence properties that enable chronic infection. Methods: 77 S. aureus isolates were obtained from 45 de-identified patients with CF at the University of Iowa. We assessed isolates phenotypically and used genotyping assays to determine the presence or absence of 18 superantigens (SAgs). Results: We observed phenotypic diversity among S. aureus isolates from patients with CF. Genotypic analysis for SAgs revealed 79.8% of CF clinical isolates carried all six members of the enterotoxin gene cluster (EGC). MRSA and MSSA isolates had similar prevalence of SAgs. We additionally observed that EGC SAgs were prevalent in S. aureus isolated from two geographically distinct CF centers. Conclusions: S. aureus SAgs belonging to the EGC are highly prevalent in CF clinical isolates. The greater prevalence in these SAgs in CF airway specimens compared to skin isolates suggests that these toxins confer selective advantage in the CF airway.
Details
- Title: Subtitle
- High Prevalence of Staphylococcus aureus Enterotoxin Gene Cluster Superantigens in Cystic Fibrosis Clinical Isolates
- Creators
- Anthony J. Fischer - Univ Iowa, Stead Family Dept Pediat, Carver Coll Med, Iowa City, IA 52242 USASamuel H. Kilgore - Roy J. and Lucille A. Carver College of MedicineSachinkumar B. Singh - Roy J. and Lucille A. Carver College of MedicinePatrick D. Allen - Roy J. and Lucille A. Carver College of MedicineAlexis R. Hansen - Roy J. and Lucille A. Carver College of MedicineDominique H. Limoli - Roy J. and Lucille A. Carver College of MedicinePatrick M. Schlievert - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Genes, Vol.10(12), p.1036
- DOI
- 10.3390/genes10121036
- PMID
- 31842331
- PMCID
- PMC6947208
- NLM abbreviation
- Genes (Basel)
- ISSN
- 2073-4425
- eISSN
- 2073-4425
- Publisher
- Mdpi
- Number of pages
- 11
- Grant note
- K08 HL136927 / NHLBI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) University of Iowa LIMOLI18F5 / CFF CFF FISCHE16I0 / Cystic Fibrosis Foundation; Italian Cystic Fibrosis Research Foundation K08HL136927 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) University of Iowa Department of Pediatrics
- Language
- English
- Date published
- 12/01/2019
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics
- Record Identifier
- 9984297326802771
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