Journal article
High-Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment
Advanced functional materials, Vol.31(1), pp.1-12
01/01/2021
DOI: 10.1002/adfm.202005021
PMCID: 8577425
PMID: 34764824
Abstract
Accurately replicating and analyzing cellular responses to mechanical cues is vital for exploring metastatic disease progression. However, many of the existing in vitro platforms for applying mechanical stimulation seed cells on synthetic substrates. To better recapitulate physiological conditions, a novel actuating platform is developed with the ability to apply tensile strain on cells at various amplitudes and frequencies in a high-throughput multi-well culture plate using a physiologically relevant substrate. Suspending fibrillar fibronectin across the body of the magnetic actuator provides a matrix representative of early metastasis for 3D cell culture that is not reliant on a synthetic substrate. This platform enables the culturing and analysis of various cell types in an environment that mimics the dynamic stretching of lung tissue during normal respiration. Metabolic activity, YAP activation, and morphology of breast cancer cells are analyzed within one week of cyclic stretching or static culture. Further, matrix degradation is significantly reduced in breast cancer cell lines with metastatic potential after actuation. These new findings demonstrate a clear suppressive cellular response due to cyclic stretching that has implications for a mechanical role in the dormancy and reactivation of disseminated breast cancer cells to macrometastases.
Details
- Title: Subtitle
- High-Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment
- Creators
- Angel Enriquez - Purdue University West LafayetteSarah Libring - Purdue University West LafayetteTyler C. Field - Purdue University West LafayetteJulian Jimenez - Purdue University West LafayetteTaeksang Lee - Purdue University West LafayetteHyunsu Park - Purdue University West LafayetteDouglas Satoski - Purdue University West LafayetteMichael K. Wendt - Purdue University West LafayetteSarah Calve - Purdue University West LafayetteAdrian Buganza Tepole - Purdue University West LafayetteLuis Solorio - Center for Cancer ResearchHyowon Lee - Purdue University West Lafayette
- Resource Type
- Journal article
- Publication Details
- Advanced functional materials, Vol.31(1), pp.1-12
- DOI
- 10.1002/adfm.202005021
- PMID
- 34764824
- PMCID
- 8577425
- NLM abbreviation
- Adv Funct Mater
- ISSN
- 1616-301X
- eISSN
- 1616-3028
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- The Advances in Medicine (AIM) grant from Cook Medical Indiana University Health CMMI 1911346; ECCS 1944480 / National Science Foundation; National Science Foundation (NSF) UL/TR002529 / Indiana CTSI from the NIH NCATS, Clinical and Translational Science Award R00CA198929; R21NS095287 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA UL1TR002529 / CTSI TL1 predoctoral fellowship
- Language
- English
- Date published
- 01/01/2021
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984460337402771
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