Journal article
High-Throughput Screening Identifies Genes Required for Candida albicans Induction of Macrophage Pyroptosis
mBio, Vol.9(4), p.e01581-18
08/21/2018
DOI: 10.1128/mBio.01581-18
PMCID: PMC6106084
PMID: 30131363
Abstract
The innate immune system is the first line of defense against invasive fungal infections. As a consequence, many successful fungal pathogens have evolved elegant strategies to interact with host immune cells. For example,
undergoes a morphogenetic switch coupled to cell wall remodeling upon phagocytosis by macrophages and then induces macrophage pyroptosis, an inflammatory cell death program. To elucidate the genetic circuitry through which
orchestrates this host response, we performed the first large-scale analysis of
interactions with mammalian immune cells. We identified 98
genes that enable macrophage pyroptosis without influencing fungal cell morphology in the macrophage, including specific determinants of cell wall biogenesis and the Hog1 signaling cascade. Using these mutated genes, we discovered that defects in the activation of pyroptosis affect immune cell recruitment during infection. Examining host circuitry required for pyroptosis in response to
infection, we discovered that inflammasome priming and activation can be decoupled. Finally, we observed that
poptosis-associated
peck-like protein containing a
ARD (ASC) oligomerization can occur prior to phagolysosomal rupture by
hyphae, demonstrating that phagolysosomal rupture is not the inflammasome activating signal. Taking the data together, this work defines genes that enable fungal cell wall remodeling and activation of macrophage pyroptosis independently of effects on morphogenesis and identifies macrophage signaling components that are required for pyroptosis in response to
infection.
is a natural member of the human mucosal microbiota that can also cause superficial infections and life-threatening systemic infections, both of which are characterized by inflammation. Host defense relies mainly on the ingestion and destruction of
by innate immune cells, such as macrophages and neutrophils. Although some
cells are killed by macrophages, most undergo a morphological change and escape by inducing macrophage pyroptosis. Here, we investigated the
genes and host factors that promote macrophage pyroptosis in response to intracellular fungi. This work provides a foundation for understanding how host immune cells interact with
and may lead to effective strategies to modulate inflammation induced by fungal infections.
Details
- Title: Subtitle
- High-Throughput Screening Identifies Genes Required for Candida albicans Induction of Macrophage Pyroptosis
- Creators
- Teresa R O'Meara - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaKwamaa Duah - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaCynthia X Guo - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaMichelle E Maxson - Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario, CanadaRyan G Gaudet - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaKristy Koselny - Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USAMelanie Wellington - Division of Pediatric Infectious Disease, University of Iowa, Iowa City, Iowa, USAMichael E Powers - Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USAJessie MacAlpine - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaMatthew J O'Meara - Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USAAmanda O Veri - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaSergio Grinstein - Department of Biochemistry, University of Toronto, Toronto, Ontario, CanadaSuzanne M Noble - Division of Pediatric Infectious Disease, University of Iowa, Iowa City, Iowa, USADamian Krysan - Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USAScott D Gray-Owen - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, CanadaLeah E Cowen - Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada leah.cowen@utoronto.ca
- Resource Type
- Journal article
- Publication Details
- mBio, Vol.9(4), p.e01581-18
- DOI
- 10.1128/mBio.01581-18
- PMID
- 30131363
- PMCID
- PMC6106084
- NLM abbreviation
- mBio
- ISSN
- 2161-2129
- eISSN
- 2150-7511
- Grant note
- R21 AI114837 / NIAID NIH HHS PJT-153403 / CIHR PJT-153177 / CIHR T32 AI060537 / NIAID NIH HHS F32 AI115947 / NIAID NIH HHS R01 AI127375 / NIAID NIH HHS PJT-148548 / CIHR F32 AI131520 / NIAID NIH HHS FDN-154288 / CIHR F32 GM114961 / NIGMS NIH HHS
- Language
- English
- Date published
- 08/21/2018
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093361002771
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