Journal article
High-Throughput Sequencing of Campylobacter jejuni Insertion Mutant Libraries Reveals mapA as a Fitness Factor for Chicken Colonization
Journal of bacteriology, Vol.196(11), pp.1958-1967
06/01/2014
DOI: 10.1128/JB.01395-13
PMCID: PMC4010991
PMID: 24633877
Abstract
Campylobacter jejuni is a leading cause of gastrointestinal infections worldwide, due primarily to its ability to asymptomatically colonize the gastrointestinal tracts of agriculturally relevant animals, including chickens. Infection often occurs following consumption of meat that was contaminated by C. jejuni during harvest. Because of this, much interest lies in understanding the mechanisms that allow C. jejuni to colonize the chicken gastrointestinal tract. To address this, we generated a C. jejuni transposon mutant library that is amenable to insertion sequencing and introduced this mutant pool into day-of-hatch chicks. Following deep sequencing of C. jejuni mutants in the cecal outputs, several novel factors required for efficient colonization of the chicken gastrointestinal tract were identified, including the predicted outer membrane protein MapA. A mutant strain lacking mapA was constructed and found to be significantly reduced for chicken colonization in both competitive infections and monoinfections. Further, we found that mapA is required for in vitro competition with wild-type C. jejuni but is dispensable for growth in monoculture.
Details
- Title: Subtitle
- High-Throughput Sequencing of Campylobacter jejuni Insertion Mutant Libraries Reveals mapA as a Fitness Factor for Chicken Colonization
- Creators
- Jeremiah G. Johnson - University of Michigan–Ann ArborJonathan Livny - Broad InstituteVictor J. DiRita - University of Michigan–Ann Arbor
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.196(11), pp.1958-1967
- Publisher
- Amer Soc Microbiology
- DOI
- 10.1128/JB.01395-13
- PMID
- 24633877
- PMCID
- PMC4010991
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Number of pages
- 10
- Grant note
- 201065201-20594; 2013-67012-21136 / U.S. Department of Agriculture's National Institute for Food and Agriculture National Institute of Allergy and Infectious Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA HHSN272200900018C / Department of Health and Human Services R01 AI069383; T32 AI007528 / National Institute of Allergy and Infectious Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) 577709; 2013-67012-21136 / NIFA; United States Department of Agriculture (USDA); National Institute of Food and Agriculture
- Language
- English
- Date published
- 06/01/2014
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696561502771
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