Journal article
High correlation of whole-body red fluorescent protein imaging and magnetic resonance imaging on an orthotopic model of pancreatic cancer
Cancer research (Chicago, Ill.), Vol.65(21), pp.9829-9833
2005
DOI: 10.1158/0008-5472.CAN-05-1548
PMID: 16267005
Abstract
We have developed genetically fluorescent orthotopic models of human pancreatic cancer. In these models, noninvasive fluorescent protein imaging (FPI) of internal primary tumors and metastatic deposits has been carried out. Whole-body tumor images are easily and inexpensively obtained using FPI, permitting both detection and quantification of tumor load. In this study, we simultaneously compared single mice with a highly fluorescent, red fluorescent protein-expressing orthotopic pancreatic cancer xenografts with both FPI and high-resolution magnetic resonance imaging (MRI). Images were acquired at multiple time points after tumor implantation in the pancreas. Indwelling pancreatic primary tumors and metastatic foci were detected by both FPI and MRI. Moreover, a strong correlation existed between images taken with these two technologies. FPI permitted rapid, high-throughput imaging without the need for either anesthesia or contrast agents. Both FPI and MRI enabled accurate imaging of tumor growth and metastasis, although MRI enabled tissue structure to be visualized as well. FPI has high resolution and is exceedingly rapid with instant image capture. We suggest a complimentary role for these two imaging modalities.
Details
- Title: Subtitle
- High correlation of whole-body red fluorescent protein imaging and magnetic resonance imaging on an orthotopic model of pancreatic cancer
- Creators
- Michael Bouvet - University of California, San DiegoJoseph Spernyak - Roswell Park Cancer InstituteMatthew H Katz - Surgical OncologyRichard V Mazurchuk - Roswell Park Cancer InstituteShinako Takimoto - University of California, San DiegoRalph Bernacki - Roswell Park Cancer InstituteYoucef M Rustum - Roswell Park Cancer InstituteAbdool R Moossa - University of California, San DiegoRobert M Hoffman - University of California, San Diego
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.65(21), pp.9829-9833
- Publisher
- American Association for Cancer Research
- DOI
- 10.1158/0008-5472.CAN-05-1548
- PMID
- 16267005
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 2005
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359949802771
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