Journal article
High-dimensional and single-cell transcriptome analysis of the tumor microenvironment in angioimmunoblastic T cell lymphoma (AITL)
Leukemia, Vol.36(1), pp.165-176
01/01/2022
DOI: 10.1038/s41375-021-01321-2
PMID: 34230608
Abstract
Angioimmunoblastic T-cell lymphoma (AITL) is an aggressive lymphoid malignancy associated with a poor clinical prognosis. The AITL tumor microenvironment (TME) is unique, featuring a minority population of malignant CD4+ T follicular helper (TFH) cells inter-mixed with a diverse infiltrate of multi-lineage immune cells. While much of the understanding of AITL biology to date has focused on characteristics of the malignant clone, less is known about the many non-malignant populations that comprise the TME. Recently, mutational consistencies have been identified between malignant cells and non-malignant B cells within the AITL TME. As a result, a significant role for non-malignant populations in AITL biology has been increasingly hypothesized. In this study, we have utilized mass cytometry and single-cell transcriptome analysis to identify several expanded populations within the AITL TME. Notably, we find that B cells within the AITL TME feature decreased expression of key markers including CD73 and CXCR5. Furthermore, we describe the expansion of distinct CD8+ T cell populations that feature an exhausted phenotype and an underlying expression profile indicative of dysfunction, impaired cytotoxicity, and upregulation of the chemokines XCL2 and XCL1.
Details
- Title: Subtitle
- High-dimensional and single-cell transcriptome analysis of the tumor microenvironment in angioimmunoblastic T cell lymphoma (AITL)
- Creators
- Joshua C. Pritchett - Department of Hematology, Mayo Clinic, Rochester, MN, USA,Zhi-Zhang Yang - Mayo Clinic in FloridaHyo Jin Kim - Mayo Clin, Dept Hematol, Rochester, MN 55905 USAJose C. Villasboas - Mayo Clinic in FloridaXinyi Tang - Department of Hematology, Mayo Clinic, Rochester, MN, USA,Shahrzad Jalali - Department of Hematology, Mayo Clinic, Rochester, MN, USA,James R. Cerhan - Department of Health Sciences Research and Epidemiology, Mayo Clinic, Rochester, MN, USA.Andrew L. Feldman - Mayo ClinicStephen M. Ansell - Department of Hematology, Mayo Clinic, Rochester, USA
- Resource Type
- Journal article
- Publication Details
- Leukemia, Vol.36(1), pp.165-176
- Publisher
- Springer Nature
- DOI
- 10.1038/s41375-021-01321-2
- PMID
- 34230608
- ISSN
- 0887-6924
- eISSN
- 1476-5551
- Number of pages
- 12
- Grant note
- Leukemia & Lymphoma Society (LLS); Leukemia and Lymphoma Society Predolin Foundation P50 CA97274 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 01/01/2022
- Academic Unit
- Epidemiology
- Record Identifier
- 9984368086402771
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