High-dose radiation with bone marrow transfer prevents neurodegeneration in an inherited glaucoma

Michael G Anderson; Richard T Libby; Douglas B Gould; Richard S Smith; Simon W. M John

doi:10.1073/pnas.0407357102

Back

High-dose radiation with bone marrow transfer prevents neurodegeneration in an inherited glaucoma

Journal article

Open access

Peer reviewed

High-dose radiation with bone marrow transfer prevents neurodegeneration in an inherited glaucoma

Michael G Anderson, Richard T Libby, Douglas B Gould, Richard S Smith and Simon W. M John

Proceedings of the National Academy of Sciences - PNAS, Vol.102(12), pp.4566-4571

03/22/2005

DOI: 10.1073/pnas.0407357102

PMCID: PMC555465

PMID: 15758074

Files and links (1)

url

https://doi.org/10.1073/pnas.0407357102View

Published (Version of record) Open Access

Abstract

Here, we show that high-dose γ-irradiation accompanied with syngeneic bone marrow transfer can confer complete protection against glaucoma in a mouse model. Because bone marrow genotype was unaltered by this procedure, it was not the causative agent. The neuroprotection is robust and highly reproducible. Glaucoma-prone DBA/2J mice received a single treatment at 5–8 weeks of age and were protected from glaucomatous retinal ganglion cell degeneration out to 14 months of age (oldest assessed). By 12–14 months, retinal ganglion cell degeneration is usually very severe and essentially complete in the majority of untreated DBA/2J mice. To assess reproducibility, three groups of mice were treated at different times, and the results were essentially the same each time. Considering all experiments, the vast majority of treated mice had no detectable glaucomatous neurodegeneration. A beneficial effect of treatment including high-dose radiation is unprecedented, and we are not aware of any other neuroprotective effects this substantial. Because of the robust protective effect, this treatment offers another tool for studying mechanisms of neuroprotection.

Biological Sciences

retinal ganlgion cell

mouse model

neuroprotection

Details

Title: Subtitle: High-dose radiation with bone marrow transfer prevents neurodegeneration in an inherited glaucoma
Creators: Michael G Anderson - The Jackson Laboratory, Bar Harbor, ME 04609
Richard T Libby - The Jackson Laboratory, Bar Harbor, ME 04609
Douglas B Gould - The Jackson Laboratory, Bar Harbor, ME 04609
Richard S Smith - The Jackson Laboratory, Bar Harbor, ME 04609
Simon W. M John - The Jackson Laboratory, Bar Harbor, ME 04609
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.102(12), pp.4566-4571
DOI: 10.1073/pnas.0407357102
PMID: 15758074
PMCID: PMC555465
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 03/22/2005
Academic Unit: Molecular Physiology and Biophysics; Ophthalmology and Visual Sciences
Record Identifier: 9984025325702771

Metrics

25 Record Views

108 Times Cited - Web of Science