Journal article
High-magnitude, virus-specific CD4 T-cell response in the central nervous system of coronavirus-infected mice
Journal of virology, Vol.75(6), pp.3043-3047
03/2001
DOI: 10.1128/JVI.75.6.3043-3047.2001
PMCID: PMC115934
PMID: 11222733
Abstract
The neurotropic JHM strain of mouse hepatitis virus (MHV) causes acute encephalitis and chronic demyelinating encephalomyelitis in rodents. Previous results indicated that CD8 T cells infiltrating the central nervous system (CNS) were largely antigen specific in both diseases. Herein we show that by 7 days postinoculation, nearly 30% of the CD4 T cells in the acutely infected CNS were MHV specific by using intracellular gamma interferon (IFN-gamma) staining assays. In mice with chronic demyelination, 10 to 15% of the CD4 T cells secreted IFN-gamma in response to MHV-specific peptides. Thus, these results show that infection of the CNS is characterized by a large influx of CD4 T cells specific for MHV and that these cells remain functional, as measured by cytokine secretion, in mice with chronic demyelination.
Details
- Title: Subtitle
- High-magnitude, virus-specific CD4 T-cell response in the central nervous system of coronavirus-infected mice
- Creators
- J S Haring - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USAL L PeweS Perlman
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.75(6), pp.3043-3047
- DOI
- 10.1128/JVI.75.6.3043-3047.2001
- PMID
- 11222733
- PMCID
- PMC115934
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Publisher
- United States
- Grant note
- AI43497 / NIAID NIH HHS P32 AI 07533 / NIAID NIH HHS NS36592 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS T32 AI007533 / NIAID NIH HHS
- Language
- English
- Date published
- 03/2001
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777347802771
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