Journal article
High-performance liquid chromatographic separation of heparin-derived oligosaccharides
Analytical biochemistry, Vol.150(2), pp.325-331
1985
DOI: 10.1016/0003-2697(85)90518-4
PMID: 4091260
Abstract
Heparin has been enzymatically depolymerized with heparinase (heparin lyase (EC 4.2.2.7)) and then separated into di-, tetra-, hexa-, octa-, and decasaccharide mixtures by low-pressure gelpermeation chromatography (GPC). These sized mixtures were resolved by strong anion-exchange (SAX) HPLC into multiple components. The fractions from the SAX-HPLC were collected and characterized for size by GPC-HPLC and sulfate content by ion chromatography. This study provides detailed methodology for the separation of larger and more highly sulfated oligosaccharides than previously reported. It describes the first use of ion chromatography for the accurate determination of the sulfate content of heparin oligosaccharides, a method which can also be applied to heparin and other glycosaminoglycans.
Details
- Title: Subtitle
- High-performance liquid chromatographic separation of heparin-derived oligosaccharides
- Creators
- K.G RiceY.S KimA.C GrantZ.M MerchantR.J Linhardt
- Resource Type
- Journal article
- Publication Details
- Analytical biochemistry, Vol.150(2), pp.325-331
- DOI
- 10.1016/0003-2697(85)90518-4
- PMID
- 4091260
- NLM abbreviation
- Anal Biochem
- ISSN
- 0003-2697
- eISSN
- 1096-0309
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1985
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065699902771
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