Journal article
High-performance liquid chromatography assay for N-acetylcysteine in biological samples following derivatization with N-(1-pyrenyl)maleimide
Journal of chromatography. Biomedical applications, Vol.685(2), pp.329-334
1996
DOI: 10.1016/S0378-4347(96)00196-X
PMID: 8953175
Abstract
N-Acetylcysteine is a thiol antioxidant with expanding clinical importance. A sensitive, rapid method for determining reduced
N-acetylcysteine (NAC) concentration in biological samples has been developed which uses a modified reversed-phase high-performance liquid chromatography (HPLC) technique in conjunction with the derivatizing agent
N-(1-pyrenyl)maleimide (NPM). The NAC-NPM adduct was analyzed by HPLC with fluorescence detection. The calibration curve for NAC was linear over the range 8–2500 n
M and the coefficient of variation obtained for the within-run precision and the between-run precision for 0.5 m
M NAC was 1.5% and 2.7%, respectively. Relative recovery of NAC from biological materials ranged between 86% and 96% and the limit of quantitation from biological samples was 32 n
M. These results suggest practical advantages relative to other widely-accepted methods of NAC measurement.
Details
- Title: Subtitle
- High-performance liquid chromatography assay for N-acetylcysteine in biological samples following derivatization with N-(1-pyrenyl)maleimide
- Creators
- Nuran Ercal - Missouri University of Science and TechnologySerdar Oztezcan - Missouri University of Science and TechnologyTerese C. Hammond - Missouri University of Science and TechnologyRichard H. Matthews - Phelps County Regional Medical CenterDouglas R. Spitz - Forest Park
- Resource Type
- Journal article
- Publication Details
- Journal of chromatography. Biomedical applications, Vol.685(2), pp.329-334
- DOI
- 10.1016/S0378-4347(96)00196-X
- PMID
- 8953175
- NLM abbreviation
- J Chromatogr B Biomed Appl
- ISSN
- 0378-4347
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1996
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312976502771
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