Journal article
High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica
Metabolic engineering, Vol.66, pp.239-258
07/01/2021
DOI: 10.1016/j.ymben.2021.04.012
PMID: 33971293
Abstract
The microalga Nannochloropsis oceanica is considered a promising platform for the sustainable production of high-value lipids and biofuel feedstocks. However, current lipid yields of N. oceanica are too low for economic feasibility. Gaining fundamental insights into the lipid metabolism of N. oceanica could open up various possibilities for the optimization of this species through genetic engineering. Therefore, the aim of this study was to discover novel genes associated with an elevated neutral lipid content. We constructed an insertional mutagenesis library of N. oceanica, selected high lipid mutants by five rounds of fluorescence-activated cell sorting, and identified disrupted genes using a novel implementation of a rapid genotyping procedure. One particularly promising mutant (HLM23) was disrupted in a putative APETALA2-like transcription factor gene. HLM23 showed a 40%-increased neutral lipid content, increased photosynthetic performance, and no growth impairment. Furthermore, transcriptome analysis revealed an upregulation of genes related to plastidial fatty acid biosynthesis, glycolysis and the Calvin-Benson-Bassham cycle in HLM23. Insights gained in this work can be used in future genetic engineering strategies for increased lipid productivity of Nannochloropsis.
Details
- Title: Subtitle
- High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica
- Creators
- Christian Sudfeld - Wageningen University & ResearchMichal Hubacek - Wageningen University & ResearchDaniel Figueiredo - Wageningen University & ResearchMihris I. S. Naduthodi - Wageningen University & ResearchJohn van der Oost - Wageningen University & ResearchRene H. Wijffels - Wageningen University & ResearchMaria J. Barbosa - Wageningen Univ, Bioproc Engn, POB 16, NL-6700 AA Wageningen, NetherlandsSarah D'Adamo - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Metabolic engineering, Vol.66, pp.239-258
- DOI
- 10.1016/j.ymben.2021.04.012
- PMID
- 33971293
- NLM abbreviation
- Metab Eng
- ISSN
- 1096-7176
- eISSN
- 1096-7184
- Publisher
- Elsevier
- Number of pages
- 20
- Grant note
- 737.016.007 / Netherlands Organization of Scientific Research (NWO) Building Blocks of Life programme; Netherlands Organization for Scientific Research (NWO) 745754 / Bio-Based Industries Joint Undertaking under the European Union's Horizon 2020 research and innovation program
- Language
- English
- Date published
- 07/01/2021
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984398032102771
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