Highly activated cytotoxic CD8 T cells express protective IL-10 at the peak of coronavirus-induced encephalitis

Kathryn Trandem; Jingxian Zhao; Erica Fleming; Stanley Perlman

doi:10.4049/jimmunol.1003292

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Highly activated cytotoxic CD8 T cells express protective IL-10 at the peak of coronavirus-induced encephalitis

Journal article

Peer reviewed

Highly activated cytotoxic CD8 T cells express protective IL-10 at the peak of coronavirus-induced encephalitis

Kathryn Trandem, Jingxian Zhao, Erica Fleming and Stanley Perlman

Journal of immunology (Baltimore, Md. : 1950), Vol.186(6), pp.3642-3652

03/15/2011

DOI: 10.4049/jimmunol.1003292

PMCID: PMC3063297

PMID: 21317392

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Abstract

Acute viral encephalitis requires rapid pathogen elimination without significant bystander tissue damage. In this article, we show that IL-10, a potent anti-inflammatory cytokine, is produced transiently at the peak of infection by CD8 T cells in the brains of coronavirus-infected mice. IL-10(+)CD8 and IL-10(-)CD8 T cells interconvert during acute disease, possibly based on recent Ag exposure. Strikingly, IL-10(+)CD8 T cells were more highly activated and cytolytic than IL-10(-)CD8 T cells, expressing greater levels of proinflammatory cytokines and chemokines, as well as cytotoxic proteins. Even though these cells are highly proinflammatory, IL-10 expressed by these cells was functional. Furthermore, IL-10 produced by CD8 T cells diminished disease severity in mice with coronavirus-induced acute encephalitis, suggesting a self-regulatory mechanism that minimizes immunopathological changes.

T-Lymphocytes, Cytotoxic - immunology

Interleukin-10 - deficiency

Mice, Inbred C57BL

Cells, Cultured

Encephalomyelitis, Acute Disseminated - prevention & control

Mice, Transgenic

Adoptive Transfer

Interleukin-10 - physiology

Coronaviridae Infections - immunology

Mice, Knockout

Murine hepatitis virus - immunology

Lymphocyte Activation - immunology

Animals

T-Lymphocytes, Cytotoxic - metabolism

T-Lymphocytes, Cytotoxic - transplantation

Coronaviridae Infections - metabolism

Encephalomyelitis, Acute Disseminated - immunology

Coronaviridae Infections - prevention & control

Inflammation Mediators - metabolism

Cell Line, Tumor

Encephalomyelitis, Acute Disseminated - pathology

Interleukin-10 - biosynthesis

Mice

Cytotoxicity, Immunologic

Details

Title: Subtitle: Highly activated cytotoxic CD8 T cells express protective IL-10 at the peak of coronavirus-induced encephalitis
Creators: Kathryn Trandem - Interdisciplinary Program in Immunology, University of Iowa, Iowa City, IA 52242, USA
Jingxian Zhao
Erica Fleming
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of immunology (Baltimore, Md. : 1950), Vol.186(6), pp.3642-3652
DOI: 10.4049/jimmunol.1003292
PMID: 21317392
PMCID: PMC3063297
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: T32 AI007511 / NIAID NIH HHS T32 AI007511-14 / NIAID NIH HHS NS36592 / NINDS NIH HHS T32 GM007337 / NIGMS NIH HHS R01 NS036592-13 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 03/15/2011
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777471102771

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