Journal article
Highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussis
Infectious diseases (London, England), Vol.48(3), pp.177-188
03/03/2016
DOI: 10.3109/23744235.2015.1100323
PMCID: PMC5278880
PMID: 26492208
Abstract
Background: Bordetella pertussis colonizes the human respiratory mucosa. Most studies on B. pertussis adherence have relied on cultured mammalian cells that lack key features present in differentiated human airway cells or on animal models that are not natural hosts of B. pertussis. The objectives of this work were to evaluate B. pertussis infection in highly differentiated human airway cells in vitro and to show the role of B. pertussis fimbriae in cell adherence. Methods: Primary human airway epithelial (PHAE) cells from human bronchi and a human bronchial epithelial (HBE) cell line were grown in vitro under air-liquid interface conditions. Results: PHAE and HBE cells infected with B. pertussis wild-type strain revealed bacterial adherence to the apical surface of cells, bacteria-induced cytoskeleton changes, and cell detachment. Mutations in the major fimbrial subunits Fim2/3 or in the minor fimbrial adhesin subunit FimD affected B. pertussis adherence to predominantly HBE cells. This cell model recapitulates the morphologic features of the human airway infected by B. pertussis and confirms the role of fimbriae in B. pertussis adherence. Furthermore, HBE cells show that fimbrial subunits, and specifically FimD adhesin, are critical in B. pertussis adherence to airway cells. Conclusions: The relevance of this model to study host-parasite interaction in pertussis lies in the striking physiologic and morphologic similarity between the PHAE and HBE cells and the human airway ciliated and goblet cells in vivo. These cells can proliferate in vitro, differentiate, and express the same genetic profile as human respiratory cells in vivo.
Details
- Title: Subtitle
- Highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussis
- Creators
- Claudia Guevara - Division of Pediatric Infectious Diseases, Vanderbilt University School of MedicineChengxian Zhang - Division of Pediatric Infectious Diseases, Vanderbilt University School of MedicineJennifer A Gaddy - Division of Infectious Diseases, Vanderbilt University School of MedicineJunaid Iqbal - Division of Pediatric Infectious Diseases, Vanderbilt University School of MedicineJulio Guerra - Division of Pediatric Infectious Diseases, Vanderbilt University School of MedicineDavid P Greenberg - Scientific and Medical Affairs, Sanofi PasteurMichael D Decker - Department of Health Policy, Vanderbilt University School of MedicineNicholas Carbonetti - Department of Biological and Biomedical Sciences, Department of Microbiology and Immunology, University of Maryland School of MedicineTimothy D Starner - Stead Family Department of Pediatrics, University of Iowa Carver College of MedicinePaul B McCray - Stead Family Department of Pediatrics, University of Iowa Carver College of MedicineFrits R Mooi - National Institute for Public Health and the Environment, Center for Infectious Diseases ControlOscar G Gómez-Duarte - Division of Pediatric Infectious Diseases, Vanderbilt University School of Medicine
- Resource Type
- Journal article
- Publication Details
- Infectious diseases (London, England), Vol.48(3), pp.177-188
- DOI
- 10.3109/23744235.2015.1100323
- PMID
- 26492208
- PMCID
- PMC5278880
- NLM abbreviation
- Infect Dis (Lond)
- ISSN
- 2374-4235
- eISSN
- 2374-4243
- Publisher
- Taylor & Francis
- Language
- English
- Date published
- 03/03/2016
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093371202771
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