Journal article
Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras
Biosensors & bioelectronics, Vol.58(100), pp.157-164
08/15/2014
DOI: 10.1016/j.bios.2014.02.042
PMCID: PMC4009403
PMID: 24632461
Abstract
Phase sensitive Surface Plasmon Resonance (SPR) techniques are a popular means of characterizing biomolecular interactions. However, limitations due to the narrow dynamic range and difficulty in adapting the method for multi-point sensing have restricted its range of applications. This paper presents a compact phase sensitive SPR technology using a custom CMOS camera. The system is exceptionally versatile enabling one to trade dynamic range for sensitivity without altering the optical system. We present results showing sensitivity over the array of better than 10(-6) Refractive Index Units (RIU) over a refractive index range of 2×10(-2)RIU, with peak sensitivity of 3×10(-7)RIU at the center of this range. We also explain how simply altering the settings of polarization components can give sensitivity on the order of 10(-8)RIU albeit at the cost of lower dynamic range. The consistent response of the custom CMOS camera in the system also allowed us to demonstrate precise quantitative detection of two Fibrinogen antibody-protein binding sites. Moreover, we use the system to determine reaction kinetics and argue how the multipoint detection gives useful insight into the molecular binding mechanisms.
Details
- Title: Subtitle
- Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras
- Creators
- Jing Wang - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United KingdomRichard J Smith - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United Kingdom. Electronic address: Richard.J.Smith@nottingham.ac.ukRoger A Light - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United KingdomJoanna L Richens - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United KingdomJing Zhang - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United KingdomPaul O'Shea - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United KingdomChung See - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United KingdomMichael G Somekh - Institute of Biophysics, Imaging and Optical Science, Department of Electrical and Electronic Engineering, University of Nottingham, Nottinghamshire NG7 2RD, United Kingdom
- Resource Type
- Journal article
- Publication Details
- Biosensors & bioelectronics, Vol.58(100), pp.157-164
- DOI
- 10.1016/j.bios.2014.02.042
- PMID
- 24632461
- PMCID
- PMC4009403
- NLM abbreviation
- Biosens Bioelectron
- ISSN
- 0956-5663
- eISSN
- 1873-4235
- Publisher
- England
- Grant note
- G1001500 / Medical Research Council
- Language
- English
- Date published
- 08/15/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Otolaryngology; Internal Medicine
- Record Identifier
- 9984006349502771
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