Journal article
HilE Interacts with HilD and Negatively Regulates hilA Transcription and Expression of the Salmonella enterica Serovar Typhimurium Invasive Phenotype
Infection and immunity, Vol.71(3), pp.1295-1305
03/2003
DOI: 10.1128/IAI.71.3.1295-1305.2003
PMCID: PMC148843
PMID: 12595445
Abstract
The ability of
Salmonella enterica
serovar Typhimurium to traverse the intestinal mucosa of a host is an important step in its ability to initiate gastrointestinal disease. The majority of the genes required for this invasive characteristic are encoded on
Salmonella
pathogenicity island 1 (SPI1), and their expression is controlled by the transcriptional activator HilA, a member of the OmpR/ToxR family of proteins. A variety of genes (
hilC
,
hilD
,
fis
,
sirA
/
barA
,
csrAB
,
phoB
,
fadD
,
envZ
/
ompR
,
fliZ
,
hilE
,
ams
,
lon
,
pag
, and
hha
) have been identified that exert positive or negative effects on
hilA
expression, although the mechanisms by which these gene products function remain relatively unclear. Recent work indicates that the small DNA-binding protein, Hha, has a significant role in repressing
hilA
transcription and the invasive phenotype, particularly in response to osmolarity signals. We have characterized the
Salmonella
-specific gene,
hilE
, and found that it plays an important regulatory role in
hilA
transcription and invasion gene expression. Mutation of
hilE
causes derepression of
hilA
transcription, and overexpression of
hilE
superrepresses
hilA
expression and the invasive phenotype. Bacterial two-hybrid experiments indicate that the HilE protein interacts with HilD, suggesting a possible mechanism for HilE negative regulation of
hilA
gene expression and the
Salmonella
invasive phenotype. Finally, we have found that the
hilE
gene resides on a region of the serovar Typhimurium chromosome that has many characteristics of a pathogenicity island.
Details
- Title: Subtitle
- HilE Interacts with HilD and Negatively Regulates hilA Transcription and Expression of the Salmonella enterica Serovar Typhimurium Invasive Phenotype
- Creators
- M. Aaron Baxter - Genetics ProgramThomas F Fahlen - Genetics ProgramRebecca L Wilson - Genetics ProgramBradley D Jones - Genetics Program
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.71(3), pp.1295-1305
- DOI
- 10.1128/IAI.71.3.1295-1305.2003
- PMID
- 12595445
- PMCID
- PMC148843
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 03/2003
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083249302771
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