Journal article
Histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) and alters HagB-induced chemokine responses
Scientific reports, Vol.4(1), pp.3904-3904
01/29/2014
DOI: 10.1038/srep03904
PMCID: PMC3912440
PMID: 24473528
Abstract
Histatins are human salivary gland peptides with anti-microbial and anti-inflammatory activities. In this study, we hypothesized that histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) and attenuates HagB-induced chemokine responses in human myeloid dendritic cells. Histatin 5 bound to immobilized HagB in a surface plasmon resonance (SPR) spectroscopy-based biosensor system. SPR spectroscopy kinetic and equilibrium analyses, protein microarray studies, and I-TASSER structural modeling studies all demonstrated two histatin 5 binding sites on HagB. One site had a stronger affinity with a KD1 of 1.9 μM and one site had a weaker affinity with a KD2 of 60.0 μM. Binding has biological implications and predictive modeling studies and exposure of dendritic cells both demonstrated that 20.0 μM histatin 5 attenuated (p < 0.05) 0.02 μM HagB-induced CCL3/MIP-1α, CCL4/MIP-1β, and TNFα responses. Thus histatin 5 is capable of attenuating chemokine responses, which may help control oral inflammation.
Details
- Title: Subtitle
- Histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) and alters HagB-induced chemokine responses
- Creators
- Derek S Borgwardt - Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAAaron D Martin - SensíQ Technologies Inc., 800 Research Parkway, Suite 100, Oklahoma City, OK 73104, USAJonathan R Van Hemert - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAJianyi Yang - Department of Computational Medicine & Bioinformatics, The University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109-2218, USACarol L Fischer - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAErica N Recker - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAPrashant R Nair - Cellworks Research India Ltd, Whitefield, Bangalore, IndiaRobinson Vidva - Cellworks Research India Ltd, Whitefield, Bangalore, IndiaShwetha Chandrashekaraiah - Cellworks Research India Ltd, Whitefield, Bangalore, IndiaAnn Progulske-Fox - Center for Molecular Microbiology and Department of Oral Biology, Box 100424, 1395 Center Drive, University of Florida, Gainesville, FL 32610, USADavid Drake - 1] Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA Department of Endodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAJoseph E Cavanaugh - Department of Biostatistics, College of Public Health, The University of Iowa, Iowa City, IA 52242, USAShireen Vali - Cellworks Group Inc., 2025 Gateway Place, San Jose, CA 95110, USAYang Zhang - University of MichiganKim A Brogden - 1] Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.4(1), pp.3904-3904
- DOI
- 10.1038/srep03904
- PMID
- 24473528
- PMCID
- PMC3912440
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- England
- Grant note
- R01 DE014390 / NIDCR NIH HHS T90 DE023520 / NIDCR NIH HHS R01 GM083107 / NIGMS NIH HHS R01 GM084222 / NIGMS NIH HHS
- Language
- English
- Date published
- 01/29/2014
- Academic Unit
- Statistics and Actuarial Science; Preventive and Community Dentistry; Endodontics; Biostatistics; Injury Prevention Research Center; Dental Research; Periodontics
- Record Identifier
- 9983985715002771
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