Histological assessment and gene expression analysis of intra‐oral soft tissue graft donor sites

Sandra Stuhr; Felipe Nör; Kareem Gayar; Emilio Couso-Queiruga; Leandro Chambrone; Iñaki Gamborena; Purnima Kumar; Gustavo Avila-Ortiz; Sukirth M. Ganesan

doi:10.1111/jcpe.13843

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Histological assessment and gene expression analysis of intra‐oral soft tissue graft donor sites

Journal article

Open access

Peer reviewed

Histological assessment and gene expression analysis of intra‐oral soft tissue graft donor sites

Sandra Stuhr, Felipe Nör, Kareem Gayar, Emilio Couso-Queiruga, Leandro Chambrone, Iñaki Gamborena, Purnima Kumar, Gustavo Avila-Ortiz and Sukirth M. Ganesan

Journal of clinical periodontology, Vol.50(10), pp.1360-1370

10/2023

DOI: 10.1111/jcpe.13843

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Abstract

Aim To determine the structural and gene expression features of different intra-oral soft tissue donor sites (i.e., anterior palate, posterior palate, maxillary tuberosity and retromolar pad). Materials and Methods Standardized mucosal tissue punch biopsies were collected from at least one donor site per subject. Histological processing was performed to determine tissue morphometry and quantify collagen composition. Site-specific gene distribution was mapped using targeted gene expression analysis and validated using real time polymerase chain reaction (qPCR). Results A total of 50 samples from 37 subjects were harvested. Epithelial thickness did not differ between sites. However, lamina propria was thicker in the maxillary tuberosity (2.55 ± 0.92 mm) and retromolar pad (1.98 ± 0.71 mm) than in the lateral palate. Type I collagen was the predominant structural protein in the lamina propria (75.06%–80.21%). Genes involving collagen maturation and extracellular matrix regulation were highly expressed in the maxillary tuberosity and retromolar pad, while lipogenesis-associated genes were markedly expressed in the lateral palate. The retromolar pad showed the most distinct gene expression profile, and the anterior and posterior palate displayed similar transcription profiles. Conclusions Tissue samples harvested from the anterior and posterior palate differed morphologically from those from the maxillary tuberosity and retromolar pad. Each intra-oral site showed a unique gene expression profile, which might impact their biological behaviour and outcomes of soft tissue augmentation procedures.

Connective Tissue

Gene Expression

Histology

Phenotype

oral mucosa

UIOWA OA Agreement

Details

Title: Subtitle: Histological assessment and gene expression analysis of intra‐oral soft tissue graft donor sites
Creators: Sandra Stuhr - University of Iowa
Felipe Nör - University of Michigan
Kareem Gayar - University of Iowa
Emilio Couso-Queiruga - University of Iowa
Leandro Chambrone - University of Pennsylvania
Iñaki Gamborena - Fomento de San Sebastián (Spain)
Purnima Kumar - University of Michigan
Gustavo Avila-Ortiz - University of Iowa
Sukirth M. Ganesan - University of Iowa
Resource Type: Journal article
Publication Details: Journal of clinical periodontology, Vol.50(10), pp.1360-1370
DOI: 10.1111/jcpe.13843
ISSN: 0303-6979
eISSN: 1600-051X
Publisher: Wiley
Grant note: DOI: 10.13039/100008893, name: University of Iowa
Language: English
Electronic publication date: 07/09/2023
Date published: 10/2023
Academic Unit: Family Dentistry; Periodontics
Record Identifier: 9984444868102771

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