Histopathologic Evaluation and Scoring of Viral Lung Infection

David K Meyerholz; Amanda P Beck

doi:10.1007/978-1-0716-0211-9_16

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Histopathologic Evaluation and Scoring of Viral Lung Infection

Journal article

Open access

Histopathologic Evaluation and Scoring of Viral Lung Infection

David K Meyerholz and Amanda P Beck

Methods in molecular biology (Clifton, N.J.), Vol.2099, pp.205-220

2020

DOI: 10.1007/978-1-0716-0211-9_16

PMCID: PMC7123785

PMID: 31883098

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https://doi.org/10.1007/978-1-0716-0211-9_16View

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Abstract

Emergent coronaviruses such as MERS-CoV and SARS-CoV can cause significant morbidity and mortality in infected individuals. Lung infection is a common clinical feature and contributes to disease severity as well as viral transmission. Animal models are often required to study viral infections and therapies, especially during an initial outbreak. Histopathology studies allow for identification of lesions and affected cell types to better understand viral pathogenesis and clarify effective therapies. Use of immunostaining allows detection of presumed viral receptors and viral tropism for cells can be evaluated to correlate with lesions. In the lung, lesions and immunostaining can be qualitatively described to define the cell types, microanatomic location, and type of changes seen. These features are important and necessary, but this approach can have limitations when comparing treatment groups. Semiquantitative and quantitative tissue scores are more rigorous as these provide the ability to statistically compare groups and increase the reproducibility and rigor of the study. This review describes principles, approaches, and resources that can be useful to evaluate coronavirus lung infection, focusing on MER-CoV infection as the principal example.

Animals

Coronavirus Infections - pathology

Coronavirus Infections - virology

Humans

Lung - pathology

Lung - virology

Mice

Middle East Respiratory Syndrome Coronavirus - physiology

Reproducibility of Results

Respiratory Tract Infections - pathology

Respiratory Tract Infections - virology

Viral Tropism

Details

Title: Subtitle: Histopathologic Evaluation and Scoring of Viral Lung Infection
Creators: David K Meyerholz - University of Iowa
Amanda P Beck - Albert Einstein College of Medicine
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.2099, pp.205-220
DOI: 10.1007/978-1-0716-0211-9_16
PMID: 31883098
PMCID: PMC7123785
NLM abbreviation: Methods Mol Biol
eISBN: 9781071602119; 107160211X
ISSN: 1064-3745
eISSN: 1940-6029
Grant note: P30 DK054759 / NIDDK NIH HHS P01 HL091842 / NHLBI NIH HHS
Language: English
Date published: 2020
Academic Unit: Pathology
Record Identifier: 9984186678602771

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