Journal article
Histopathologic Technique for the Morphological Examination of Fixed or Frozen Solid Tumor Cells in Soft Agar
Biotechnic & histochemistry, Vol.56(6), pp.349-353
1981
DOI: 10.3109/10520298109067342
PMID: 6176051
Abstract
Two simple techniques are described for preparing sections from soft agar colony cultures of tumor cells. Tumor cells grown in soft agar can be frozen, sectioned, and stained and/or fixed in formalin, embedded in paraffin, sectioned, mounted on glass slides, and stained. The methods are simple and reproducible. These cells can be stained with various stains and the staining quality is excellent. The paraffin blocks and microscope slides can be stored for permanent record. The use of these techniques should provide better understanding of the histomorphologic characteristics of neoplastic cells which grow in soft agar and should expand and refine prognosis and diagnosis of malignant tumors.
Details
- Title: Subtitle
- Histopathologic Technique for the Morphological Examination of Fixed or Frozen Solid Tumor Cells in Soft Agar
- Creators
- Z. P. Pavelic - Roswell Park Cancer InstituteM. M. Vaughan - Roswell Park Cancer InstituteH. K. Slocum - Roswell Park Cancer InstituteY. M. Rustum - Roswell Park Cancer Institute
- Resource Type
- Journal article
- Publication Details
- Biotechnic & histochemistry, Vol.56(6), pp.349-353
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/10520298109067342
- PMID
- 6176051
- ISSN
- 1052-0295
- eISSN
- 1473-7760
- Language
- English
- Date published
- 1981
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359886302771
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