Journal article
Homophilic Protocadherin Cell-Cell Interactions Promote Dendrite Complexity
Cell reports (Cambridge), Vol.15(5), pp.1037-1050
05/03/2016
DOI: 10.1016/j.celrep.2016.03.093
PMCID: PMC4856576
PMID: 27117416
Abstract
Growth of a properly complex dendrite arbor is a key step in neuronal differentiation and a prerequisite for neural circuit formation. Diverse cell surface molecules, such as the clustered protocadherins (Pcdhs), have long been proposed to regulate circuit formation through specific cell-cell interactions. Here, using transgenic and conditional knockout mice to manipulate γ-Pcdh repertoire in the cerebral cortex, we show that the complexity of a neuron’s dendritic arbor is determined by homophilic interactions with other cells. Neurons expressing only one of the 22 γ-Pcdhs can exhibit either exuberant or minimal dendrite complexity, depending only on whether surrounding cells express the same isoform. Furthermore, loss of astrocytic γ-Pcdhs, or disruption of astrocyte-neuron homophilic matching, reduces dendrite complexity cell non-autonomously. Our data indicate that γ-Pcdhs act locally to promote dendrite arborization via homophilic matching, and they confirm that connectivity in vivo depends on molecular interactions between neurons and between neurons and astrocytes.
[Display omitted]
•Increasing γ-Pcdh homophilic matching promotes dendrite complexity in the cortex•Inducing γ-Pcdh homophilic mismatching reduces dendrite complexity in the cortex•Astrocytes regulate dendrite complexity cell non-autonomously via γ-Pcdh interactions•γ-Pcdhs promote dendrite complexity through local interactions
Molumby et al. manipulate the expression of γ-Protocadherin adhesion molecule isoforms in the mouse cerebral cortex to demonstrate that the complexity of a neuron’s dendritic arbor is promoted by local homophilic interactions with other neurons and with astrocytes.
Details
- Title: Subtitle
- Homophilic Protocadherin Cell-Cell Interactions Promote Dendrite Complexity
- Creators
- Michael J Molumby - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAAustin B Keeler - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAJoshua A Weiner - Department of Biology, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Cell reports (Cambridge), Vol.15(5), pp.1037-1050
- DOI
- 10.1016/j.celrep.2016.03.093
- PMID
- 27117416
- PMCID
- PMC4856576
- NLM abbreviation
- Cell Rep
- ISSN
- 2211-1247
- eISSN
- 2211-1247
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 05/03/2016
- Academic Unit
- Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983997989902771
Metrics
23 Record Views