Journal article
Hormonal Regulation of Luteinizing Hormone/Chorionic Gonadotropin Receptor mRNA in Rat Ovarian Cells during Follicular Development and Luteinization
Molecular endocrinology (Baltimore, Md.), Vol.4(12), pp.1856-1865
12/1990
DOI: 10.1210/mend-4-12-1856
PMID: 2127956
Abstract
To determine whether hormone-dependent changes in the levels of LH/CG receptor in the rat ovary are associated with changes in expression of LH/CG receptor mRNAs, total RNA from rat follicles and corpora lutea at various stages of development was prepared and analyzed by Northern blots and/or solution hybridization. Whereas small antral follicles contained lo amounts of LH/CG receptor mRNAs, the growth of preovulatory (PO) follicles was associated with an increase in all LH/CG receptor mRNA transcripts. Induction of LH/CG receptor mRNAs in granulosa cells of hypophysectomized rats was dependent on the synergistic effects of estradiol and FSH. An LH/CG surge in vivo or LH treatment of PO follicles in vitro caused a rapid decline of all LH/CG receptor mRNAs in PO follicles, which was prevented by cycloheximide. Newly formed corpora lutea (days 1-4 postovulation) contained low amounts of LH/CG receptor mRNAs unless the rats were pregnant or treated exogenously with PRL. During pregnancy, corpora lutea isolated on days 4-19 of gestation contained high levels of LH/CG receptor mRNAs, which decreased markedly on days 21 and 24, the time of functional luteolysis and decreasing LH/CG receptor levels. These studies demonstrate that hormonal regulation of LH/CG receptor mRNA in rat ovarian cells parallels changes in LH/CG receptor levels and involves diverse molecular mechanisms, including 1) low concentrations of cAMP (elicited by FSH) in developing follicles, 2) inhibition by high concentrations of cAMP (elicited by LH/CG) in PO follicles, and 3) induction and maintenance by PRL in corpora lutea of gestation.
Details
- Title: Subtitle
- Hormonal Regulation of Luteinizing Hormone/Chorionic Gonadotropin Receptor mRNA in Rat Ovarian Cells during Follicular Development and Luteinization
- Creators
- Deborah L SegaloffHaiyun WangJoAnne S Richards
- Resource Type
- Journal article
- Publication Details
- Molecular endocrinology (Baltimore, Md.), Vol.4(12), pp.1856-1865
- DOI
- 10.1210/mend-4-12-1856
- PMID
- 2127956
- NLM abbreviation
- Mol Endocrinol
- ISSN
- 0888-8809
- eISSN
- 1944-9917
- Language
- English
- Date published
- 12/1990
- Academic Unit
- Molecular Physiology and Biophysics; Obstetrics and Gynecology
- Record Identifier
- 9984083861102771
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